外源性胆绿素对中波紫外线诱导的角质形成细胞光损伤的保护作用和机制

【摘要】 目的 探讨外源性胆绿素对中波紫外线（UVB）照射的HaCaT细胞光损伤的保护作用。方法 将HaCaT细胞分为加入0、0.1、1、10 μmol/L胆绿素并照射UVB的UVB组、0.1 μmol/L UVB组、1 μmol/L UVB组、10 μmol/L UVB组及不做处理的对照组。UVB照射剂量为30 mJ/cm2，照射后继续培养24 h，分别检测细胞活性氧（ROS）水平、超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量，ELISA法检测各组细胞的炎症因子白细胞介素6（IL-6）、IL-8水平。多组间均数比较采用单因素方差分析，组间两两比较采用LSD-t检验。结果 UVB组、0.1 μmol/L UVB 组、1 μmol/L UVB组、10 μmol/L UVB组、对照组细胞ROS水平（3 613.33 ± 206.61、2 958.67 ± 193.87、2 678.33 ± 178.24、2 274.67 ± 118.81、1 905.67 ± 250.25）、SOD活力（24.41 ± 1.78、28.96 ± 2.21、29.75 ± 1.75、30.19 ± 2.29、37.52 ± 2.31）、MDA含量（5.61 ± 0.32、5.46 ± 0.55、4.65 ± 0.22、2.55 ± 0.93、1.31 ± 0.05）、IL-6水平、IL-8水平差异均有统计学意义（F值分别为 34.02、57.36、214.09、29.73、11.40，均P < 0.05），UVB组ROS水平、MDA含量及IL-6、IL-8水平均高于另4组（均P < 0.05），SOD活力均低于另4组（均P < 0.05）。结论 外源性胆绿素减轻UVB引起的HaCaT细胞的氧化损伤、减轻炎症反应和抑制脂质过氧化作用，对细胞光损伤有一定的保护作用。

Protective effect of exogenous biliverdin on ultraviolet B-induced photodamage to keratinocytes and its mechanisms

【Abstract】 Objective To evaluate the protective effect of exogenous biliverdin on ultraviolet B （UVB）-induced photodamage to keratinocytes, and to explore its mechanisms. Methods HaCaT cells were divided into 5 groups: UVB group irradiated with 30 mJ/cm2 UVB alone, 0.1-, 1- and 10-μmol/L UVB groups treated with 0.1, 1, 10 μmol/L biliverdin respectively and irradiated with 30 mJ/cm2 UVB, and control group receiving no treatment. After irradiation, cells in the above groups continued to be cultured for 24 hours. Then, the reactive oxygen species （ROS） level, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content were detected in HaCaT cells, and enzyme-linked immunosorbent assay （ELISA） was performed to detect levels of the inflammatory factors interleukin 6 （IL-6） and IL-8 in HaCaT cells. One-way analysis of variance was used for comparison of means among multiple groups, and least significant difference （LSD）-t test was used for multiple comparisons. Results Significant differences were observed among the UVB group, 0.1-, 1- and 10-μmol/L UVB groups and control group in the ROS levels （3 613.33 ± 206.61, 2 958.67 ± 193.87, 2 678.33 ± 178.24, 2 274.67 ± 118.81, 1 905.67 ± 250.25, respectively, F = 34.02, P < 0.05）, SOD activity （24.41 ± 1.78, 28.96 ± 2.21, 29.75 ± 1.75, 30.19 ± 2.29, 37.52 ± 2.31, respectively, F = 57.36, P < 0.05）, MDA contents （5.61 ± 0.32, 5.46 ± 0.55, 4.65 ± 0.22, 2.55 ± 0.93, 1.31 ± 0.05, respectively, F = 214.09, P < 0.05）, and levels of IL-6 (F = 29.73, P < 0.05) and IL-8 (F = 11.40, P < 0.05). The UVB group showed significantly increased levels of ROS, IL-6 and IL-8, and MDA content compared with the other 4 groups （all P < 0.05）, but significantly decreased SOD activity compared with the other 4 groups （P < 0.05）. Conclusion Exogenous biliverdin has some protective effect on UVB-induced photodamage, likely by reducing oxidative damage to cells, attenuating inflammatory reactions and suppressing lipid peroxidation.