miRNA-296在胃黏膜癌前病变中的表达及临床意义

目的 探讨miRNA-296的成熟体miR-296-5p和miR-296-3p在胃黏膜癌前病变中的表达及胆汁酸对其的调控作用。方法 根据病理学检查结果,将182例患者石蜡包埋胃组织样本制成的组织芯片分为4组:慢性浅表性胃炎(chronic superficial gastritis,CSG)组、慢性萎缩性胃炎(chronic atrophic gastritis,CAG)组、肠上皮化生(intestinal metaplasia,IM)组、异型增生(dysplasia,Dys)组。用原位杂交的方法检测各组miR-296-5p和miR-296-3p的表达。采用实时定量聚合酶链反应(PCR)技术检测鹅去氧胆酸(CDCA)刺激人胃黏膜上皮细胞系GES-1细胞后miR-296-5p和miR-296-3p的表达水平。结果 miR-296-5p/3p在CSG与CAG组织中的表达无统计学差异,而在IM、Dys组织中的表达均明显降低。经CDCA刺激人胃黏膜上皮细胞株GES-1细胞后,miR-296-5p和miR-296-3p的表达水平明显降低。结论 miR-296-5p/3p在IM及Dys病变组织中表达明显低于CSG中表达,胆汁酸抑制胃上皮细胞表达miR-296,这可能是胆汁酸诱导胃黏膜发生癌变的机制。

The expression and chinical sigificance of miRNA-296 in gastric mucosal precancerous lesions

Objective To investigate the expression of miR-296-5p and miR-296-3p,the mature bodies of miRNA-296 in gastric mucosal precancerous lesions and the regulation of bile acids on them. Methods In the tissue chip,they were divided into 4 groups according to the pathological results:chronic superficial gastritis(CSG)group,chronic atrophic gastritis(CAG)group,intestinal metaplasia(IM)group,and dysplasia(Dys)group.In situ hybridization was used to detect the expression of miR-296-5p and miR-296-3p in each group.Real-time quantitative polymerase chain reaction (PCR) technology was used to detect the expression levels of miR-296-5p and miR-296-3p after bile acid CDCA stimulated human gastric mucosal epithelial cell line GES-1 cells. Results The expression rate of miR-296-5p/3p was not statistically different in CSG and CAG tissues,but the expression in IM and Dys tissues was significantly reduced.After CDCA stimulated human gastric mucosal epithelial cell line GES-1 cells,the expression levels of miR-296-5p and miR-296-3p were significantly reduced. Conclusion The expression of miR-296-5p/3p in gastric mucosa IM and Dys is significantly lower than that in CSG.Bile alids inhibit the expression of miR-296 in gastnc epithelial cells,which may be the mechanism by which bile acids induce precancerous lesions in gastric mucosa.