翻白草总黄酮的制备及抗炎活性研究

目的优化翻白草总黄酮制备工艺,并探讨其抗炎活性。方法翻白草经70%乙醇提取、脱醇,上聚酰胺柱进行纯化,以芦丁为对照,分光光度法检测翻白草总黄酮在各工艺单元的分布及纯度;以脂多糖(LPS)诱导单核巨噬细胞RAW264.7,建立炎症筛选模型,用不同浓度(25、50、100μg·mL^-1)的翻白草总黄酮处理后,定量PCR法检测细胞炎症因子一氧化氮合酶(iNOS)、IL-1β及IL-6的mRNA表达水平。结果聚酰胺层析所得翻白草总黄酮纯度为87.4%,回收率为82.6%;实验剂量(25、50、100μg·mL^-1)下,翻白草总黄酮可显著下调LPS诱导的RAW 264.7细胞炎症因子iNOS、IL-1β及IL-6的mRNA的表达。结论翻白草总黄酮可用聚酰胺树脂层析制备,其抗炎活性可通过下调iNOS、IL-1β、IL-6 mRNA的表达来实现。

Study on preparation process and anti-inflammatory activity of total flavonoids of PotentilladiscolorBge.

Objective To study on the preparation process and anti-inflammatory activity of total flavonoids of PotentilladiscolorBge. Methods The plant was extracted with 70% ethanol, and purified with polyamide resin, and rutin was used as a control to investigate the distribution and purity of total flavones.Mononuclear macrophage RAW264.7 was induced with lipopolysaccharide (LPS), and after the treatment with different concentrations (25, 50, 100 μg·mL^-1) of total flavonoids of PotentilladiscolorBge., quantitative PCR was used to detect the mRNA expression levels of inflammatory factors iNOS, IL-1β, and IL-6. Results The purity of total flavonoids of PotentilladiscolorBge. obtained by polyamide chromatography was 87.4%, and the yield was 82.6%. The total flavones of PotentilladiscolorBge. could significantly down-regulate the mRNA expression of inflammatory factors iNOS, IL-1β and IL-6 in RAW 264.7 cells induced by LPS. Conclusion The total flavonoids of PotentilladiscolorBge. can be prepared by polyamide resin chromatography, and its anti-inflammatory activity is related to the down-regulation of mRNA expression of iNOS, IL-1β, and IL-6.