阿魏酸通过调控线粒体凋亡抑制宫颈癌HeLa细胞侵袭迁移功能

目的：探讨阿魏酸（FA）对宫颈癌HeLa细胞株凋亡调控作用及其可能机制。方法：体外培养HeLa细胞，将其分为control组和FA处理组（1、2 和4 mmol/L）。细胞计数试剂盒8（CCK-8）和划痕实验检测细胞增殖和迁移能力，Transwell实验检测细胞的侵袭能力。实时定量聚合酶链反应（RT-qPCR）检测不同浓度FA处理后HeLa细胞凋亡相关基因Bcl-2、Bax及Cleaved-caspase-3的表达，免疫荧光检测HeLa细胞中线粒体的表达数量及状态。结果：RT-qPCR结果显示，与control组相比，随着FA浓度增高，HeLa细胞中Bcl-2基因表达逐渐降低，而Bax和Cleaved-caspase-3 基因表达逐渐增强（P ＜0.05）。免疫荧光示，与control组相比，HeLa细胞中的线粒体含量逐渐减少且功能逐渐减弱（P ＜0.05）。Transwell以及划痕实验结果显示，与control组相比，不同浓度FA可使HeLa细胞的侵袭细胞数以及迁移细胞数减少且呈剂量依赖性（P ＜0.05）。结论：FA通过Bcl-2家族蛋白途径抑制HeLa细胞的增殖、侵袭及迁移，诱导线粒体介导的细胞凋亡。

Ferulic acid inhibits the invasion and migration of cervical cancer HeLa cells by regulating mitochondrial apoptosis in vitro

Objective: To study the effect of Ferulic Acid (FA) on cervical cancer (HeLa) cell lines and its possible mechanism. Methods: HeLa cells were cultured in vitro and divided into the control group and FA treatment group (1, 2 and 4 mmol/L). CCK-8 and scratch test detected cell proliferation and migration ability.Transwell test evaluated cell invasion ability. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of apoptosis-related genes Bcl-2, Bax and Cleaved-caspase-3 in HeLa cells after treatment with different concentrations of FA, and immunofluorescence was used to detect the expression and status of mitochondria in HeLa cells. Results: RT-qPCR results showed that the expression of Bcl-2 gene in HeLa cells gradually decreased, while the expression of Bax and Cleaved-caspase-3 genes gradually increased in a dose-dependent manner, with statistical difference (P<0.05), compared with the control group.Immunofluorescence showed that the content of mitochondria in HeLa cells gradually decreased and the function gradually weakened with statistical difference (P<0.05), compared with the control group. The results of Transwell and scratch experiments showed that different concentrations of FA reduced the number of invaded cells and migrated cells of HeLa cells in a dose-dependent manner with significant differences (P<0.05),compared with the control group. Conclusion: FA inhibits the proliferation, invasion and migration of HeLa cells through the Bcl-2 family protein pathway, and induces mitochondrial-mediated apoptosis.