IL-12B基因启动子区异常甲基化与卵巢子宫内膜异位症的发病风险相关性研究

目的探讨白细胞介素12B(interleukin-12B,IL-12B)基因启动子区甲基化状态及其mRNA在异位内膜和正常内膜组织中的表达水平，分析其在卵巢子宫内膜异位症（endometriosis，EMs）中的作用。 方法应用焦磷酸测序和实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction，qRT-PCR）技术分别检测50例卵巢EMs患者异位内膜组织和44例对照妇女正常内膜组织中IL-12B基因启动子区甲基化水平和mRNA的表达情况。 结果与正常内膜组织相比，卵巢EMs患者异位内膜组织中IL-12B基因启动子区呈现明显的低甲基化状态(P＜0.001)，而mRNA的表达水平则显著高于对照妇女正常内膜组织(P＜0.001)。相关性分析显示组织中IL-12B基因启动子区甲基化水平与其mRNA表达水平呈明显负相关(r=-0.510,P＜0.001)。 结论IL-12B基因启动子区异常甲基化水平导致mRNA表达水平的改变可能在卵巢EMs的发生发展中起重要作用。

Correlation of abnormal methylation of the IL-12B gene promoter region with the risk of ovarian endometriosis

ObjectiveTo investigate the methylation status of interleukin-12B（IL-12B） gene promoter region and its mRNA expression level in ectopic endometrium and normal endometrium, and to analyze its role in ovarian endometriosis.MethodsA pyrosequencing assay was used to analyse the methylation levels of IL-12B gene promoter region in ectopic endometrium of 50 patients with role in ovarian endometriosis and normal endometrium of 44 control women. The mRNA expression of IL-12B gene was detected by quantitative real-time PCR(qRT-PCR).ResultsThe methylation levels of the IL-12B gene promoter region in ectopic endometrium were significantly lower than those in normal endometrium(P＜0.001). mRNA levels of IL-12B gene were significantly increased in ectopic endometrium compared to those in normal endometrium(P＜0.001). Correlation analysis showed that the methylation levels of the IL-12B gene promoter region were negatively correlated with its mRNA levels(P＜0.001).ConclusionThe aberrant methylation of the IL-12B gene promoter region may play an important role in the occurrence and development of ovarian endometriosis by affecting its mRNA expression level.