LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药

目的验证长链非编码RNA SOX2OT(Long noncoding RNA SOX2OT,SOX2OT)增强大肠癌HCT-116细胞多药耐药(Multidrug resistance,MDR),并从SOX2OT/miR-34a/SOX2信号通路探讨其可能的有效机制。方法双荧光素酶报告基因验证SOX2OT与miR-34a、miR-34a与SOX2的靶向结合;HCT-116细胞瞬时转染SOX2OT质粒、miR-34a mimic,RT-qPCR验证转染效率;RT-qPCR检测SOX2OT、miR-34a和SOX2表达水平并进行相关性分析;CCK-8检测细胞增殖,评估SOX2OT、miR-34a对HCT-116细胞5-氟尿嘧啶(5-FU)、长春新碱(VCR)、顺铂(CDDP)、紫杉醇(TAXOL)化疗敏感性的影响。结果 SOX2OT与miR-34a、miR-34a与SOX2之间存在靶向关系;SOX2OT抑制miR-34a(P<0.05),上调SOX2(P<0.01);miR-34下调SOX2OT(P<0.01)和SOX2(P<0.01);转染SOX2OT质粒后HCT...

LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer

Objective The aim of this study was to verify that long noncoding RNA SOX2OT(SOX2OT)enhanced the multidrug resistance(MDR)of colorectal cancer HCT-116 cells,and explored its possible effective mechanism on SOX2OT/miR-34a/SOX2 signaling pathway.Methods Dual luciferase reporter gene assay was used to verify the targeted binding of SOX2OT to miR-34a,and miR-34a to SOX2;The SOX2OT plasmid and miR-34a were transient transfected into HCT-116 cells,and the transfection efficiency was verified by RT-qPCR;RT-qPCR was used to detect the levels of SOX2OT,miR-34a and SOX2,and the correlation amongst them were analyzed;The CCK-8 assay was used to detect cell proliferation and to assess the influence of SOX2OT and miR-34a on the chemosensitivity of HCT-116 cells to 5-fluorouracil(5-FU),vincristine(VCR),cisplatin(CDDP),and paclitaxel(Taxol).Results There was a targeting relationship between SOX2OT and miR-34a,and miR-34a with SOX2;The SOX2OT inhibited miR-34a(P＜0.05)and upregulated the level of SOX2(P＜0.01);The MiR-34a down-regulated the expression of SOX2OT and SOX2(P＜0.01);The IC₅₀ values for 5-FU,VCR,CDDP,and TAXOL in HCT-116 cells were increased after transfection of SOX2OT plasmid(P＜0.01);The IC₅₀ values of four chemotherapy drugs in the co-transfected with SOX2OT and miR-34a mimic groups were significantly lower than those of the transfected SOX2OT group(P＜0.01).The IC₅₀ values of 5-FU and CDDP were not different from the control group(P＞0.05).Conclusion The SOX2OT sponge adsorbs miR-34a and promotes the expression of SOX2,resulting in multidrug resistance of HCT-116 cells.