| 生物活性产品玻璃化冷冻保存研究进展 |
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| 引用本文： | 秦廷武（综述） 莫湘涛（综述） 杨志明（审校）. 生物活性产品玻璃化冷冻保存研究进展[J]. 生物医学工程学杂志, 2005, 22(5): 1070-1074 |
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| 作者姓名： | 秦廷武（综述） 莫湘涛（综述） 杨志明（审校） |
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| 作者单位： | 四川大学华西医院生物治疗国家重点实验室干细胞和组织工程研究室 成都610041(秦廷武，莫湘涛)，四川大学华西医院生物治疗国家重点实验室干细胞和组织工程研究室 成都610041(杨志明) |
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| 基金项目： | 国家自然科学基金资助项目(30070199,30570469)，四川省青年科技基金资助项目(2001-19-0132) |
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| 摘 要： | 综述了有关动物细胞、胚胎、组织、器官和工程化组织的玻璃化冷冻保存问题。对玻璃化冻存技术基本原理及其在生物产品保存中的冷冻、复温方法,玻璃化液的组成、作用及其对保存产品的影响等进行了阐述。指出玻璃化冷冻保存可能是长时间保存有活性的生物产品简单、适用、有效的方法。同时指出玻璃化冷冻保存方法面临的问题及发展的方向。
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| 关 键 词： | 玻璃化 冷冻保存 生物产品 |
| 收稿时间： | 2003-12-17 |
| 修稿时间： | 2003-12-172004-03-10 |
Progress in Vitreous Cryopreservation of Living Bioproducts |
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| Qin Tingwu, Mo Xiangtao, Yang Zhiming. Progress in Vitreous Cryopreservation of Living Bioproducts[J]. Journal of biomedical engineering, 2005, 22(5): 1070-1074 |
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| Authors: | Qin Tingwu Mo Xiangtao Yang Zhiming |
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| Affiliation: | Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu 610041,China |
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| Abstract: | In this brief review, some key issues related to vitreous cryopreservation of living tissues (natural or engineered), including cells, embryos, tissues, organs, and engineered tissues, are outlined. The principle of vitreous cryopreservation for the biological activity and functionality is demonstrated. The procedures of cooling/rewarming, composition and function of optimal cryoprotectants, and their effects on bioproducts are described. Vitrification could, therefore, prove to be a useful and effective method of bioproduct cryopreservation for a long period of time, particularly for organized tissues and organs. However, the toxicity of the cryoprotective agents and the devitrification occurring during the rewarming process need additional investigations. Several key areas of research on vitrification are also addressed. |
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| Keywords: | Vitrification Cryopreservation Bioproducts |
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