The relationship between bcl-2 gene abnormality and nasopharyeal carcinoma

Bcl--2/JHfusiongenecausedbyt(14;18)(q32;q21)mayleadtoaberrantexpressionofhcf--2geneandbecloselyrelatedtogenesisoflymphomaL'~':.Inordertoexploretherelationshipbetweenhcf--2geneaberrantandnasopharyngealcarcinoma(NPC),hcf--2/JHfusiongeneandhcf--2proteinexpressionwereexaminedwithsemi--nestedinsituPCR(SNISPCR)andimmunohistochemistrytechniquein41casesofNPC.MATERIALSANDMETHODSTissuepreparationFortyonesampleswereobtainedbybiopsyfrom41patientsinourdepartmentfromApril1996toMarch1997.Oft…

The relationship between bcl-2 gene abnormality and nasopharyeal carcinoma

objective To explore the relationship between bcl--2 gene abnormality and nasopharyngeal carcinoma (NPC) Methods: bcl--2/JH fusion gene and bcl-2 protein expression were examined with semi--nested insitu PCR (SNISPCR) and immunohistochemistry technique in 41 NPC. Results: (l) Bcl-2/JH fusion genewere found in 6 cases of 41 NPC (positivity rate, 14. 6% ). (2)Bol-2 protein was expressed in 34 cases of 41NPC, the positivity rate being 82. 9 %. No bcl-2 expression was seen in benign nasopharyngeal lesions. Conclusion: (l) Translocation was not a specific change in lymphoma, but mbr and mcr regions are the two important breakpoint regions in NPC. Bcl-2/JH fusion gene formation may not play an important role in pathogenesis of NPC for its low positive rate. (2) There was no corresponding relationship between formation ofhcf-2/JH fusion gene and aberrant hcf-2 protein expression in NPC.