自身免疫甲状腺病单个核细胞体外培养产生抗甲状腺球蛋白抗体

利用ELISA技术检测自身免疫甲状腺病(AITD)患者周围血单个核细胞(PBMC)体外培养产生甲状腺球蛋白抗体(TGA)。结果表明:体外培养AITD患者PBMC能够产生可测出的TGA,阳性率31.25％。桥本甲状腺炎(HT)较Graves甲亢(GD)更易体外产生TGA,阳性率分别为44.19％和16.22％。美洲商陆刺激体外产生TGA增加,而可溶性甲状腺球蛋白对体外产生TGA无刺激作用。抗甲状腺药物他巴唑能够抑制体外产生TGA。HT患者血清TGA水平与体外产生TGA呈正相关,但未观察到GD患者血清TGA与体外产生TGA的相关性。提示AITD患者体内存在可引起自身抗体产生的免疫调节紊乱、HT与GD在产生自身抗体的机制上可能有所不同。

THYROGLOBULIN ANTIBODY PRODUCTION BY CULTURED PERIPHERAL BLOOD MONONUCLEAR CELLS FROM PATIENTS WITH AUTOIMMUNE THYROID DISEASE

Thyroglobulin antibody (TGA) production in vitro by cultured peripheral blood mononuclear cells (PBMC) from patients with autoimmune thyroid disease (AITD) was measured by ELISA technique. PBMC from patients with AITD could synthesize and secret detectable amount of TGA. The average positive rate of TGA production in vitro was 31.25%. PBMC from the patients with Hashimoto thyroiditis (HT) could produce TGA more easily than that from the patients with Graves'disease (GD). The average positive rate was 44.19% in HT and 16.22% in GD. Pokeweed mitogen could increase TGA production, but soluable thyroglobulin could not stimulate TGA synthesis and secretion. Antithyroid drug methi-mazole had some inhibition effect on TGA production in vitro. A significant positive correlation between the serum TGA and the amount of TGA produced in vitro by PBMC from HT patients (r = 0.4, P<0.005) was observed, but from GD patients no such correlation could be found. It suggested that immunoregulation disorders in autoantibody production resulted in the patients with AITD. The mechanism of autoantibody production was different between HT and GD patients.