| RP-HPLC测定民族药材白花丹不同药用部位中白花丹醌的含量 |
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| 引用本文： | 刘圆,邓放,刘超,孟庆艳,高泽文.RP-HPLC测定民族药材白花丹不同药用部位中白花丹醌的含量[J].中国中药杂志,2006,31(20):1684-1686. |
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| 作者姓名： | 刘圆 邓放 刘超 孟庆艳 高泽文 |
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| 作者单位： | 1. 西南民族大学,少数民族药物研究所,四川,成都,610041
2. 成都中医药大学,药学院,四川,成都,610075 |
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| 基金项目： | 四川省科技攻关项目;西南民族大学校科研和教改项目 |
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| 摘 要： | 目的：建立白花丹药材中白花丹醌的含量测定方法并考察白花丹不同药用部位中的含量。方法：色谱条件：Kromasil C18柱(4.6 mm×200 mm,5 μm),柱温30 ℃。流动相甲醇-水（65∶35）,检测波长213 nm,流速1 mL·min-1。结果：白花丹醌在0.020 8～0.104 μg,峰面积与进样量具有良好的线性关系,回归方程Y=-14.29+9 138.9 X,r= 0.999 9,平均回收率为98.7%。白花丹各部位中白花丹醌的含量分别为：根0.394%,茎0.050%,叶0.031%。结论：本法简便、准确、重复性好,可用于控制白花丹药材质量。
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| 关 键 词： | 白花丹 白花丹醌 含量 RP-HPLC |
| 文章编号： | 1001-5302（2006）20-1684-03 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005-06-28 |
Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC |
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| LIU Yuan ; DENG Fang ;LIU Chao ;MENG Qing-yan ;GAO Ze-wen.Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC[J].China Journal of Chinese Materia Medica,2006,31(20):1684-1686. |
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| Authors: | LIU Yuan ; DENG Fang ;LIU Chao ;MENG Qing-yan ;GAO Ze-wen |
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| Institution: | Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To develop a RP-HPLC method to determine plumbagin in Plumbago zeylanica, and to investigate contents of plumbagin in different parts of. P. zeylanica. METHOD: The analysis was carried out at 30 degrees C on a Kromasil C18, column eluted with a mobile phase consisting of a mixture of methanol-water (65: 35). The flow rate was 1 mL x min(-1), the detector wavelength was 213 nm. RESULT: The calibration curve was linear within the concentration ranges of 0.020 8-0. 104 microg (r = 0. 9999). The average recovery was 98.7%. The contents in the root, stem and leaf were 0.394 5%, 0.050 8%, 0.031 4% respectively. CONCLUSION: This method is simple, accurate, replicate and suitable for the determination of plumbagin in P. zeylanica. |
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| Keywords: | Plumbago zeylanica plumbagin assay RP- HPLC |
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