氟尿嘧啶联合黄芪对小鼠胃癌组织氨基酸代谢的影响

目的 研究氟尿嘧啶联合中药黄芪对3-甲基胆蒽（MC）诱发的小鼠胃癌氨基酸代谢的影响规律。方法 建立MC诱发小鼠胃癌的模型，分别设立氟尿嘧啶治疗组（A组）、氟尿嘧啶加黄芪治疗组（B组）、氟尿嘧啶加高剂量黄芪治疗组（C组）、胃癌对照组（D组）、假手术组（P组）和正常组（N组），对各组标本进行病理检测，并测定胃癌标本游离氨基酸含量。结果 MC诱发3个月时小鼠胃癌发癌率为48．7％。P组胃组织游离氨基酸中缬氨酸、蛋氨酸、亮氨酸、精氨酸和胱氨酸含量高于正常组织（P〈0．05），且与D组无明显差异（P〉0．05）。A、B、C、D组中的丝氨酸含量高于N组（P〈0．05）；A、B组谷氨酸含量明显高于P、N组（P〈0．05），且A、B、C、D组的数值呈逐渐下降的趋势。C、D组胃癌组织中脯氨酸含量明显高于P、N组（P〈0．05），且A、B、C组的数值呈逐渐升高的趋势。讨论 MC诱发的小鼠胃癌模型中丝氨酸、脯氨酸升高可反映其氨基酸代谢紊乱；氟尿嘧啶与黄芪联合应用，可减少胃癌组织中谷氨酸含量而抑制肿瘤生长。

Effect of 5-flurouracil in combination with astragalus membranaceus on amino acid metabolism in mice model of gastric carcinoma

OBJECTIVE: To study the effect of 5-flurouracil-FU in combination with astragalus membranaceus(AM) on amino acid metabolism in mice model of gastric carcinoma induced by 3-methylcholanthrene(MC). METHODS: Mice gastric carcinoma models were established by 3-methylcholanthrene induction and randomly divided into different groups, and received 5-FU treatment (group A) 5-FU plus AM (group B), 5-FU plus a high dose of AM(group C), no treatment (group D). Normal mice were used as control (group N). Free amino acid in the tumor specimens were examined. RESULTS: The levels of free Valine, Methionine, Leucine, Arginine and cystine in the tumor specimens in group D were significantly higher than that in group N(P< 0.05). The levels of free serine in group A, B, C, D were significantly higher than that in group N. The levels of free glutamic acid in group A, B were significantly higher than that in group N(P< 0.05). The levels of free proline in group C, D were significantly higher than that in group P, N(P< 0.05). CONCLUSIONS: The increasing levels of free serine and proline in tumor specimens in gastric cancer mice model reveals metabolic disturbance of amino acid. 5-FU plus astragalus membranaceus can decrease the level of free glutamic acid in the mice models, and inhibit tumor growth.