亚麻木酚素联合硼替佐米诱导肺腺癌A549细胞凋亡的机制研究

目的:探讨亚麻木酚素(SDG)联合蛋白酶体抑制剂硼替佐米(Bortezomib,Bor)对肺腺癌A549细胞凋亡的影响及其机制。方法:MTT法检测细胞增殖;Annexin V-FITC/PI双染流式细胞法和Hoechst 33258荧光染色法检测细胞凋亡;比色法检测半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)活性;Real Time PCR检测Caspase-3、BCL-2、BAXmRNA的表达;Western Blot检测BCL-2、Bax及p-JNK的蛋白表达。结果:SDG联合Bor能明显抑制细胞生长,上调Caspase-3活性,促进p-JNK、BAX mRNA和蛋白的表达,抑制BCL-2 mRNA和蛋白的表达,诱导细胞凋亡。结论:SDG联合Bor可显著诱导肺腺癌A549细胞的凋亡,其机制可能与其激活JNK信号通路有关。

Effects of secoisolariciresinol diglucoside combined with bortezomib on induction of apoptosis in Lung Cancer Cell Line A549

Objective: To study the influence of Secoisolariciresinol Diglucoside(SDG) combined with Bortezomib on induction of apoptosis in Lung Cancer Cell Line A549 and its relative mechanisms.Methods: The effect on proliferation was evaluated by MTT assay.The cell apoptosis was studied by flow cytometry and Hoechst 33342 staining.Colorimetric method was used to detect the activity of Caspase-3.Real Time PCR was used to detect the expression of Caspase-3,BCL-2 and BAX mRNA.Western blot was used to determine the change of p-JNK,BCL-2 and BAX protein expression in A549 cells.Results: The cell growth was significantly slowed down and the cell apoptosis was induced after the combined treatment.Meanwhile the Caspase-3 activity and the expression of Caspase-3 mRNA were obviously increased,the expression of BCL-2 mRNA and protein were significantly down regulated and the expression of BAX.p-JNK mRNA and protein were significantly up regulated after the combined treatment.Conclusion: The results demonstrate that SDG combined with Bortezomib can significantly induce apoptosis of A549 cells,its mechanisms may be involved in activation of the JNK pathway.