响应曲面法优化无患子油超临界CO_2萃取工艺研究及其成分分析

目的:研究无患子油超临界CO2萃取工艺及其成分分析。方法:以无患子为材料,采用响应曲面法优化无患子油超临界CO2萃取工艺,并采用GC-MS对无患子油成分进行分析。结果:建立了一个能较好预测萃取结果的数学模型,并根据该方程对无患子油的超临界萃取工艺参数进行了优选。确定了无患子油超临界CO2萃取最佳工艺为:萃取压力30 MPa,萃取温度40℃,分离Ⅰ压力14 MPa、温度45℃,分离Ⅱ压力6 MPa、温度40℃,萃取时间60 min,得率为17.58%;并对无患子油进行了GC-MS分析,鉴定了22种成分,其中不饱和脂肪酸含量为86.59%。结论:优化后的工艺合理可行,操作简单,可用于无患子油的提取。

Optimization for supercritical CO2 extraction with response surface methodology and component analysis of Sapindus mukorossi oil

Objective:To study the extraction conditions of Sapindus mukorossi oil by Supercritical CO2 Extraction and identify its components.Methods:Optimized SFE-CO2 Extraction by Response Surface Methodology and used GC-MS to analysie Sapindus mukorossi oil compounds.Results:Established the model of an equation for the extraction rate of Sapindus mukorossi oil by Supercritical CO2 Extraction,and the optimal parameters for the Supercritical CO2 Extraction determined by the equation were:the extraction pressure was 30 MPa,temperature was 40 ℃;The separationⅠ pressure was 14 MPa,temperature was 45 ℃;The separationⅡ pressure was 6 MPa,temperature was 40 ℃;The extraction time was 60 min and the extraction rate of Sapindus mukorossi oil of 17.58%.22 main compounds of Sapindus mukorossi oil extracted by supercritical CO2 were identified by GC-MS,unsaturated fatty acids were 86.59%.Conclusion:This process is reliable,safe and with simple operation,and can be used for the extraction of Sapindus mukorossi oil.