杜仲叶桃叶珊瑚苷的酶法提取及其抑菌活性

目的:研究杜仲叶桃叶珊瑚苷的酶法提取工艺及其抑菌活性。方法:采用纤维素酶法,以桃叶珊瑚苷提取率为指标,优选最佳提取条件,并对其抑菌效果进行研究。结果:杜仲叶桃叶珊瑚苷的最佳提取工艺为:料液比1∶12,酶解pH 6.0,酶用量0.4%,酶解温度50℃,酶解时间50 min,溶出量可达17.892 mg/g。桃叶珊瑚苷提取液对大肠杆菌和金黄色葡萄球菌的抑菌作用较强,对金黄色葡萄球菌的MIC为4.832 mg/mL,对大肠杆菌的MIC为9.664 mg/mL,但对肺炎链球菌和MG溶血性链球菌的抑菌作用不明显,MIC均为28.946 mg/mL。结论:该提取工艺合理可行,提取率高,提取得到的桃叶珊瑚苷具有一定的抑菌作用。

Enzymatic extraction and antibacterial activity of aucubin from Eucommia ulmoides leaves

Objective:To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity.Methods:Aucubin in Eucommia ulmoides leaves was extracted by cellulase method.The extraction technology was optimized using the content of Aucubin as index.The antibacterial activity of Aucubin was determined.Results:The results showed that the optimum technology was as follows;The solid-liquid ratio was 1∶12,extracted for 50 min by 0.4% enzyme at 50 ℃ in pH 6.0.The extraction rate of Aucubin was as high as 17.892 mg/g.The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus,the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively.However,Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus,the MIC of Aucubin against them were all 28.946 mg/mL.Conclusion:The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate,and the Aucubin has some antibacterial activity.