短葶山麦冬愈伤组织诱导与多糖积累动态研究

目的:研究比较短葶山麦冬茎尖、叶片、块根和果实等不同外植体诱导发生愈伤组织的成功率及多糖积累动态状况。方法:比较2,4-D、蔗糖不同浓度及光照条件等对短葶山麦冬茎尖愈伤组织诱导及愈伤组织继代保持的影响,并对愈伤组织多糖含量动态变化及愈伤组织、试管苗和非试管苗块根中多糖进行了比较研究。结果与结论:以茎尖为外植体愈伤组织诱导率高;2,4-D浓度为1.5～2.0 mg/L时有利于短葶山麦冬茎尖诱导愈伤组织,诱导率达87.5%。添加0.5～1.0 mg/L 2,4-D、20 g/L蔗糖时,茎尖愈伤组织的增殖系数最大,且愈伤组织形态和生长势良好。光照条件不影响愈伤组织的继代保持。茎尖愈伤组织中的多糖含量随培养时间的延长而增加,超过60d后,愈伤组织中多糖含量趋于稳定;培养40～60 d后多糖含量较高,此时愈伤组织生长达到最高速率。短葶山麦冬试管苗产生的块根中多糖含量高于非试管苗块根,愈伤组织多糖含量最低,但其生产多糖的速度较其他2种方式快。

Callus induction of Liriope muscari and its dynamic accumulation of polysaccharide

Objective: To study the induction rate of callus tissue in four different explants of stem,root,leaf and fruit of Liriope muscari.Methods: The effect of 2,4-D,sugar and illumination on callus succeeding preservation was analyzed.The dynamic accumlation of polysaccharide in callus was described.The polysaccharide content among callus,tissue culture seedings and field seedings was compared.Results and conclusion: The callus induction rate of stem was the highest.The optimal concentration range of 2,4-D was 1.5~2.0 mg/L,then the induction rate was 87.5%.When the 2,4-D conncentration was 0.5~1.0 mg/L,and the sucrose concentration was 20 g/L,the multiplication coefficient was highest.The illumination condition did not influence the effect of callus succeeding preservation.The content of callus polysaccharide continuously increased for 60 d.The growth rate of callus was reached the highest level from 40 d to 60 d.Polysaccharide content in root of tissue culture seeding was higher than that of the field seeding.