不同居群蒙古扁桃的等位酶变异及遗传多样性分析

目的:在等位酶水平上对蒙古扁桃4个不同野生居群的遗传多样性进行分析。方法:采用聚丙烯酰胺凝胶垂直平板电泳技术。结果:多态位点百分率P=77.78%,等位基因平均数A=1.7916,平均观察杂合度(Ho)=0.4009,平均期望杂合度(He)=0.4898;蒙古扁桃居群间的遗传分化系数(GST)为0.0693,说明其6.93%的遗传变异来自于居群间;基于遗传分化系数计算的基因流(Nm)为3.3576,说明居群间存在适度的基因流;固定系数(F)为0.0896,显示蒙古扁桃居群纯合体轻微过量,杂合体略显不足。结论:蒙古扁桃种群仍具有较高的遗传多样性并且在适宜的生境中可以完成自然更新,应采取就地保护的保育措施。

The analysis of allozymic variation and genetic diversity in different Prunus mongolica population

Objective:To evaluate the allozymic variation and genetic diversity from four wild population of Prunus mongolica.Methods:The technique of polyacrylamide gel electrophoresis was used.Results:The percentage of loci polymorphic(P) was 77.78%.The mean number of alleles per locus(A) was 1.7916.The mean observed heterozygosity per locus(H0) was 0.4009 and the mean expected heterozygosity per locus(He) was 0.4898.The coefficient of gene differentiation(GST) was 0.0693 which explained that 6.93% of the total genetic diversity was distributed within populations.The gene flow(Nm) was 3.3575,which indicated the gene flow took place among populations.The fixation index(F) was 0.0896 which indicated a slight excess of homozygote within populations and a little insufficiency of heterozygosity.Conclusion:Considering the relatively high level of genetic diversity in Prunus mongolica regenerating in the largest population,we recommend in situ conservation to maintain viable Prunus mongolica populations in their original habitats.