睡眠剥夺对大鼠海马和前额皮层N-甲基-D-天冬氨酸受体mRNA表达的影响

目的观察不同睡眠剥夺时间对海马和前额皮层N-甲基-D-天冬氨酸受体(NMDAR)mRNA表达的影响,为进一步阐明睡眠剥夺损害认知功能的可能机制提供实验依据。方法雄性Wistar大鼠随机分为3组,即24 h、48 h和72 h实验组,每组再分为睡眠剥夺组(SD组)和对照组(C组)。利用睡眠剥夺箱建立大鼠SD模型,Trizol一步法提取海马和前额皮层总RNA,用SYBRAgreenⅠ荧光定量RT-PCR测定NMDAR的3个亚单位,即NR1、NR2A和NR2B mRNA水平。结果(1)与相应时间的对照组相比,大鼠海马NR1 mRNA表达水平在24 h和48 h SD后均有降低的趋势,72 h SD后下降65.7%(P0.05);NR2AmRNA水平在48 h SD后有降低的趋势,72 h SD后下降53.0%(P0.05);在24 h、48 h和72 h SD后,海马NR2B mRNA水平与对照组比无统计学差异。(2)与相应时间的对照组相比,大鼠前额皮层NR1和NR2A mRNA水平在48 h和72 h SD后都表现出降低趋势,其中NR2A mRNA在72 h SD后下降60.3%(P0.05);在24 h、48 h和72 hSD后,前额皮层NR2B mRNA水平都未发生改变。结论睡眠剥夺可以使大鼠海马和前额皮层NR1及NR2A mRNA表达水平降低,且随着睡眠剥夺时间的延长,降低更为明显,这可能是睡眠剥夺损害认知功能的原因之一。

EFFECTS OF SLEEP DEPRIVATION ON N-METHYL-D-ASPARTATE RECEPTOR mRNA EXPRESSION IN HIPPOCAMPUS AND FOREBRAIN OF RATS

Objective To study the effects of different duration of sleep deprivation(SD) on N-methyl-D-aspartate receptor(NMDAR) mRNA expression in hippocampus and forebrain,so as to provide experimental basis for further clarifying the possible mechanisms of SD damaging to cognitive functions.Methods Male Wistar rats were divided into three groups: 24 h,48 h and 72 h experimental group.Each of them was subdivided into sleep deprivation group(SD group) and control group(C group).SD model was established by sleep depri...