实验性自身免疫性脑脊髓炎大鼠血-脑脊液屏障功能及动态变化

目的　探讨实验性自身免疫性脑脊髓炎 ( EAE)大鼠血 -脑脊液屏障功能的动态变化及其作用 ,以求进一步揭示 EAE发病机制。方法　检测 EAE大鼠免疫后第 4、6、8、1 0、1 2、1 4、1 6、1 8、2 0天血清和脑脊液 ( CSF)中清蛋白 ( ALB)含量 ,其 CSF与血清 ALB比值 ( QA)作为评价血 -脑脊液屏障损害的指标。结果　 QA值免疫后第 8天即出现显著性升高 ,早于临床症状出现 ;随免疫时间的延长 QA值呈逐渐增高后缓慢下降趋势 ,并且 QA值与EAE大鼠病情评分呈显著正相关 ( r =0 .81 ,P =0 .0 0 1 )。结论　血 -脑脊液屏障的早期损害在 EAE发病过程中具关键作用。

The Kinetic and Effect Study on Blood-brain Barrier Function during Experimental Autoimmune Encephalomyelitis in Rats

Objective To investigate the kinetic changes and effect of blood brain barrier function during experimental autoimmune encephalomyelitis (EAE) in rats. Methods Albumin in serum and CSF samples of EAE rats were examined on the days of 4, 6, 8, 10, 12, 14, 16, 18 and 20 p.i.. The integrity of blood brain barrier was assessed by calculating CSF to serum albumin quotient (QA). Results The increase of QA on day 8 p.i. was prior to development of clinical symptoms. QA of EAE rats showed dynamic changes with days p.i. and there was a markedly positive correlation between clinical scores and QA (r=0 81,P=0 001). Conclusions Blood brain barrier damage during the earlier period of EAE might play an important role in the pathogenesis of EAE.