| 高效液相色谱法测定人血浆中加替沙星浓度 |
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| 引用本文： | 裴奇,刘世坤,李佐军,周于禄.高效液相色谱法测定人血浆中加替沙星浓度[J].中国药房,2006,17(2):121-123. |
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| 作者姓名： | 裴奇 刘世坤 李佐军 周于禄 |
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| 作者单位： | 1. 中南大学湘雅三医院药剂科,长沙市,410013;中南大学药学院,长沙市,410013
2. 中南大学湘雅三医院药剂科,长沙市,410013 |
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| 摘 要： | 目的:建立以高效液相色谱法测定人血浆中加替沙星浓度的方法。方法:色谱柱为C18,流动相为乙腈-30mmol/L醋酸铵-三乙胺-磷酸(20∶80∶1.0∶0.7),流速为1.0ml/min,检测波长为294nm。结果:加替沙星检测浓度在0.1～10.0μg/ml范围内线性关系良好(r=0.9992),最低检测浓度为0.05μg/ml;高、中、低系列浓度的日内RSD<8%,日间RSD<10%;平均回收率为(101.67±3.06)%。结论:本方法操作简便、灵敏、准确,适用于加替沙星的血药浓度监测及其药动学研究。
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| 关 键 词： | 加替沙星 高效液相色谱法 血药浓度 |
| 文章编号： | 1001-0408(2006)02-0121-03 |
| 收稿时间： | 02 1 2005 12:00AM |
| 修稿时间： | 03 24 2005 12:00AM |
Content Determination of Gatifloxacin in Human Plasma by HPLC |
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| PEI Qi,LIU Shikun,LI Zuojun,ZHOU Yulu.Content Determination of Gatifloxacin in Human Plasma by HPLC[J].China Pharmacy,2006,17(2):121-123. |
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| Authors: | PEI Qi LIU Shikun LI Zuojun ZHOU Yulu |
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| Abstract: | OBJECTIVE: To establish a HPLC method for content determination of gatifloxacin in human plasma.METHODS: The Analytical column was C18,the mobile phase consisted of acetonitrile-30mmol/L ammonium acetate-triethylamine-orthophosphoric acid (20∶80∶1.0∶0.7) with a flow rate of 1.0ml/min,the detection was performed at UV 294nm.RESULTS: The calibration curve was linear in the concentrations ranging from 0.1 to10.0μg /ml (r=0.9 992).The detection limit was 0.05μg/ml.The intraday RSD was less than 8% and interday RSD was less than 10%.The average recovery was (101.67±3.06)%.CONCLUSION: The method is simple, sensitive, accurate and suitable for determination of gatifloxacin in human plasma and pharmacokinetic study. |
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| Keywords: | Ggatifloxacin HPLC Plasma drug concentration |
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