乙脑单克隆抗体被动血凝抑制试验在乙脑血清学诊断上的应用

本文应用乙脑单克隆抗体(McAb)致敏羊血球(M-RBC)作反问被动血凝抑制试验(RPHI),检测临床诊断为乙脑的60份急性患者血清抗体,并与HI试验进行比较。结果表明,RPHI法阳性率为85.0%,HI试验法阳性率为53.3%。经统计分析,以RPHI法敏感性高、特异性强、方法简便和诊断快速,适于国内基层医院和卫生防疫站在开展临床诊断和流行病学调研时推广应用。

An Application of Japanese B Encephalitis McAb For Reverse Passive Hemagglutination Test in the Sero-Diagnosis Of JE

The Sauitary and Anti-epidmic Station of Zhengzhon City An Application of JaPanese B encephalitis (JE) McAb for RPHI test compared with HI test in the detection for JE antibodies of 60 serum samples of acute stage JE patients was reported. The results showed that Positive rate of RPHI was (51/60) 85.0% and HI was (32/60) 53.3%. There wao significunt difference between these two methods. The RPHI was sensitive, specific rapid and simple to perform. Therefore, this technique wao suitable for the laboratory diagnosis and serum-epidemiological study of JE in the peripheral hospitals and Anti-epidemic stations.