| 哮喘豚鼠气道内NK-1R mRNA表达及其含量的研究 |
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| 引用本文： | 李淼,尚云晓.哮喘豚鼠气道内NK-1R mRNA表达及其含量的研究[J].中国医科大学学报,2005,34(6):502-503. |
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| 作者姓名： | 李淼 尚云晓 |
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| 作者单位： | 中国医科大学附属第二医院儿科,辽宁,沈阳,110004 |
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| 摘 要： | 目的:研究哮喘豚鼠气道内神经激肽1受体(NK-1R)mRNA的分布及相对含量.方法:用卵蛋白超声雾化法制作哮喘豚鼠模型;原位杂交技术检测NK-1R mRNA,光镜观察其在气道内表达的部位.图像分析测定NK-1R mRNA的相对含量.结果:哮喘组NK-1R mRNA表达程度明显高于正常组;NK-1RmRNA分布于气管上皮细胞上层细胞表面、黏膜下层、血管周围上皮细胞、炎性细胞的表面和平滑肌细胞、腺体细胞表面.结论:NK-1R参与了卵蛋白诱导的哮喘豚鼠发病机制.
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| 关 键 词： | 哮喘 豚鼠 神经激肽1受体 |
| 文章编号： | 0258-4646(2005)06-0502-01 |
| 修稿时间： | 2004年11月16 |
Expression and content of neurokinin-1 receptor mRNA in the airway of asthmatic guinea pigs |
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| LI Miao,SHANG Yun-xiao.Expression and content of neurokinin-1 receptor mRNA in the airway of asthmatic guinea pigs[J].Journal of China Medical University,2005,34(6):502-503. |
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| Authors: | LI Miao SHANG Yun-xiao |
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| Abstract: | Objective: To study the expression and content of neurokinin-1 receptor(NK-1R) mRNA in the airway of asthmatic guinea pigs.Methods:The model of asthmatic guinea pigs was established by aerosolized ovalbumin.The expression of NK-1R mRNA was detected by in situ hybridization,and the expression site was observed under the light microscope.The content of NK-1R mRNA was determined by image analysis.Results:The expression of NK-1R mRNA in asthma group was higher than that in normal control group.NK-1R mRNA was expressed on the cell surface of tracheal epithelia,submucosa,blood vessels epithelia,inflammatory cells,smooth muscle cells,and gland cells.Conclusion:NK-1R may be involved in the pathogenesis of ovalbumin-induced asthma in guinea pigs. |
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| Keywords: | asthma guinea pig neurokinin-1 receptor |
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