尿酸减轻6-羟基多巴胺对PC12细胞的毒性作用

目的：评价尿酸减轻6．羟基多巴胺（6-OHDA）对PC12细胞的毒性作用。方法：应用PCI2细胞制作帕金森细胞模型，分为对照组、尿酸组、6-OHDA组、尿酸＋6-OHDA组。采用MTT测定各组PC12细胞活性，免疫荧光法观察各组PCI2细胞caspase-3激活情况，流式细胞术检测各组PC12细胞凋亡率。尿酸100～400μmol·L^-1不影响PCI2细胞生存率，尿酸100～400μmol·L-1可显著提高6-OHDA50gmol-L。作用6、12和24h造成的PCI2细胞生存率的下降（P〈0．01）；尿酸能减少6-OHDA导致的PCI2细胞caspase-3激活，降低6-OHDA导致的凋亡率（P〈0．05）。结论：尿酸具有减轻6-OHDA对PC12细胞的毒性作用。

Protective Effect of Uric Acid on the Cell Damage of PC12 Cells Induced by 6-OHDA

Aim： To investigate the protective effect of uric acid（UA） on the injury of PC12 cells induced by 6-hydroxydopamine（6-OHDA）. Methods： PC12 cells injured by 6-OHDA were used as the Parkinson＇ s disease cell models, which were divided into four groups： control group, UA group, 6-OHDA group and UA＋6-OHDA group. The cell viability were assesseded by MTT method. The apoptosis of cells were assessed by observing the caspase-3 protein level by using immunocytochemistry, and calculating apoptosis rate using flow cytometry（FCM） technique. Results： 50 μmol·L-1 of 6-OHDA decreased the viability of PC12 cells at 6, 12, 24 h. 100-400μmol·L-1 of UA had no significant effect on the viability of PC12 cells. 100-400μmol·L-1 of UA reversed the decease of PC 12 cells induced by 6-OHDA. The caspase-3 protein level and apoptosis rate of PC12 cell were increased after 6-OHDA（50 ~tmol-L 1） treatment for 24 h, which were decreased by UA（200 μmol·L-1） treatment （P〈0.05）. Conclusion： 6-OHDA can decrease the viability of PC 12 cells and increase the apoptosis of PC12 cells. UA can protect PC12 cells from injury induced by 6-OHDA.