RNAi抑制EGFR基因增强卵巢癌细胞对泰素敏感性的研究

目的 探讨表皮生长因子受体(EGFR)基因在卵巢癌细胞对泰素敏感性的增强作用.方法 构建针对EGFR基因序列特异性shRNA的表达载体,用脂质体转染卵巢癌SKOV3细胞及G418筛选阳性克隆.采用RT-PCR、Western blot检测EGFR基因的抑制情况,用MTT法检测卵巢癌细胞对泰素的敏感性.结果 靶向EGFR的序列特异性shRNA可明显抑制EGFR基因的表达,EGFR mRNA和蛋白的抑制率分别为77.5%和76.1%;序列特异性shRNA-EGFR可明显提高卵巢癌细胞对泰素的敏感性,差异有统计学意义(P<0.01).结论 体外实验证实抑制EGFR基因的表达可提高卵巢癌细胞的化疗敏感性.

Study of EGFR gene repressed by RNAi on enhancing chemosensitivity of ovarian cancer cells to paclitaxel

Objective To investigate the enhancement effects of epidermal growth factor receptor gene on the chemosensitivity of ovarian cancer cells to paclitaxel.Methods To construct the EGFR gene sequence-specific shRNA expression vector,and to use lipofectamine transfer ovarian cancer SKOV3 cells and G418 selected positive clones.The expression of EGFR gene was detected by RT-PCR and Western blot,the chemosensitivity of ovarian cancer cells to paclitaxel was detected by MTT assay.Results Sequence-specific shRNA tar...