骨肉瘤中细胞周期素D1,p16蛋白表达的研究

目的；研究细胞周期素Ｄ１，ｐ１６蛋白在骨肉瘤中的表达状态及意义。方法：采用免疫组织化学方法（ＳＰ法）检测了４３例骨肉瘤和１５例骨软骨瘤中２种蛋白的表达，并在２种肿瘤间及骨肉瘤患者不同年龄组，组织类型，分化程度间进行比较。

A STUDY ON EXPRESSION OF Cyclin D_1 AND p16 PROTEIN IN OSTEOSARCOMA

Objective:To investigate the expression level and action of cyclin D 1 and p16 protein in osteosarcoma.Methods:Expression of two kinds of protein 43 osteosarcoma and 15 osteochondroma was detected by immunohistochemistry staining(SP).The comparison was made among the groups with different age,histoclassification and different differentiating extent in osteosarcoma.Results:The over expression of cyclin D 1 can be observed in osteosarcoma(46.7%)(P<0.05).The positive rate (69.8%) of p16 protein in osteoscarcoma(20%) was higher than that in the osteochondroma(46.7%)(P<0.01).The positive rate of the two kinds of protein in osteosarcoma group in age,histoclassification and differentiating extent showed no significant difference.Conclusion:The abnormal expression of cyclin D 1 and p16 protein existed in osteosarcoma,and it played an important role in the occurrence and development of osteosarcoma.