| NASBA和RPA两种等温扩增技术在病原菌检测中的应用研究 |
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| 引用本文： | 岳苑,张建中,张茂俊. NASBA和RPA两种等温扩增技术在病原菌检测中的应用研究[J]. 中华流行病学杂志, 2019, 40(8): 1018-1022 |
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| 作者姓名： | 岳苑 张建中 张茂俊 |
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| 作者单位： | 中国疾病预防控制中心传染病预防控制所, 北京 102206;宁夏回族自治区食品检测研究院, 银川 750001,中国疾病预防控制中心传染病预防控制所, 北京 102206,中国疾病预防控制中心传染病预防控制所, 北京 102206 |
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| 基金项目： | 国家重点基础研究发展计划（2013CB127204）;"西部之光"人才培养项目 |
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| 摘 要： | 依赖核酸序列扩增和重组酶聚合酶扩增技术是在PCR基础上发展起来的等温扩增技术。本文介绍了这两个技术的反应原理、引物探针设计原则以及优势,根据其简便、准确性好、灵敏度高、周期短的特点进一步对该技术在病原菌检测中的应用予以综述及展望。
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| 关 键 词： | 病原菌 依赖核酸序列扩增 重组酶聚合酶扩增 |
| 收稿时间： | 2018-12-05 |
Application of NASBA and RPA in detection of pathogenic bacteria |
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| Yue Yuan,Zhang Jianzhong and Zhang Maojun. Application of NASBA and RPA in detection of pathogenic bacteria[J]. Chinese Journal of Epidemiology, 2019, 40(8): 1018-1022 |
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| Authors: | Yue Yuan Zhang Jianzhong Zhang Maojun |
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| Affiliation: | Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Ningxia Hui Autonomous Region Food Testing and Research Institute, Yinchuan 750001, China,Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China and Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Nucleic acid sequence-based amplification and recombinase polymerase amplification are the recently developed thermostatic amplification techniques based on PCR. This paper briefly summarizes the principle of reaction, design principle of primer and probe, advantage of these two techniques (simple, accurate, highly sensitive and rapid) and introduces the application of the techniques in the detection of pathogenic bacteria. |
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| Keywords: | Pathogenic bacteria Nucleic acid sequence-based amplification Recombinase polymerase amplification |
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