热休克、PMA对体外培养人角朊细胞表达ENA的影响

ENA在角朊细胞膜表达被认为与 LE的发病有关。为了探讨热休克和 PMA对 ENA表达的影响 ,我们用加热和 PMA刺激体外培养角朊细胞 ,用间接免疫荧光法观察了角朊细胞 ENA的表达。结果 ,4 2℃、4 5℃热休克 90 min后 ,分别可使 9.1± 1.2 %、2 0 .0± 4 .7%的细胞出现 ENA膜表达 ;5μg/ L、10 μg/ L PMA刺激 2 h后 ,2 2 .6± 7.6%、2 3.1± 4 .9%的角朊细胞膜出现 ENA表达。提示 ENA在角朊细胞膜的表达与热刺激和蛋白激酶 C传导路径有关。

Effect of Heat Shock,Phorbol- 12 - Myristate- 13- Acetate( PMA) on the Expression of ENA on Cul- tured Human Keratinocytes

ENA expression on the surface of the keratinocytes is considered to be associated with the patho- genesis of lupus erythematosus.In order to investigate the effect of heat shock and PMA on the expres- sion of ENA,cultured keratinocyte was treated with heat shock and PMA in vitro and the expression of ENA was determined by indirect immunofluorenscence( IIF) .The results showed that 4 2℃ and 4 5℃ heat shock tor 90 minutes induced 9.1± 1.2 % and 2 0 .0± 4 .7% of keratinocytes to express ENA on the cell membranes,respectively.Treatment of keratinocytes with PMA ( 5μg/ L、10μg/ L ) induced the ex- pression of ENA on keratinocytes( 2 2 .6± 7.6% and2 3.1± 4 .9% ,respectively) .These rdsults indicated that ENA expression on the surfacd of keratinocytes could be induced by heat stimulation and its regu- latory pathway may partly involve the protein kinase C signal transduction system.