不同变异类型药用大黄筛选及其产量和内在品质比较

目的 纯化药用大黄Rheum officinale种质,比较传统型和不同变异类型药用大黄产量和内在品质差异,为药用大黄栽培及种质选择提供依据.方法 根据药用大黄外观形态制定不同变异类型标准,于采收期测定不同类型药用大黄产量,UPLC法测定游离蒽醌、结合蒽醌、鞣质和二蒽酮等14种功效成分含量.结果 药用大黄分为传统型和3...

Screening of different variation types from Rheum officinale and comparison of their yield and intrinsic quality

Objective To purify the germplasm and reveal the yield and intrinsic quality differences of different variation types of Rheum officinale and to provide basis for germplasm selection. Methods The criterion of various varieties of R. officinale plants was set and the growth and yield were measured at the harvest stage. UPLC was used to measure the content of 14 functional components including 5 free anthraquinones, 5 binding anthraquinones, 2 tannins and 2 dianthraquinones. Results A total of three variation types were selected, named as palmately middle split type (B1), brown stem and brown vein type (B2) and brown stem and green vein type (B3). Compared with traditional type, the yield of the three variation types was lower. There were significant differences in the contents of 14 functional components (P < 0.05). Compared with traditional type, the contents of total free anthraquinone and total binding anthraquinone of the three variation types were lower, but the contents of total dianthraquinone were higher, and total tannin content of B2 type was significantly higher than that of traditional type. According to the content and proportion of efficacy components, these three variation types of R. officinale may have better purgation efficacy than the traditional type, but the efficacy of"clearing heat and detoxifing" may be lower, and the efficacy of "activate blood circulation to dissipate blood stasis" of B2 type may be higher than the traditional type. Conclusion The yield and index component of traditional type were superior to the three variation types, hence the germplasm purification is worth consideration in the cultivation of R officinale. The efficacy bias of different variation types of R. officinale provide references for its germplasm screening.