三氧化二砷对人肺腺癌A549/R细胞多药耐药相关蛋白表达的影响

目的探讨三氧化二砷(As2O3)对人肺腺癌A549/R细胞耐药性的逆转作用及对多药耐药相关蛋白（MRP）表达的影响。方法以荧光分光光度计测定细胞内药物浓度的改变，采用半定量逆转录聚合酶联反应(RT PCR) 技术检测As2O3处理后A549/R MRP基因表达的变化。结果As2O3的非细胞毒性剂量可增加A549/R细胞内多柔比星(ADM)浓度，降低其IC50。A549/R细胞中MRP呈过表达状态，不同浓度的As2O3处理A549/R后MRP表达水平明显降低。结论As2O3可部分逆转A549/R细胞对ADM的耐药性，其逆转机制与改变MRP基因表达有关。

Effect of Arsenic Tioxide on MRP Expression in A549/R Cell Line of Human Lung Cancer

Objective To investigate the effect of arsenic trioxide(As2O3) on the reversal role of drug resistance and the expression of MRP in human lung cancer cell line A549/R. Methods The comcentration of intracellular ADM was detected by fluorescence spectrofluorometry.The expression of MRP gene was assessed in As2O3-treated A549/R cells by semiq-uantitative RT-PCR. Results As2O3 in noncytotoxic dose could significantly increase the intracellular accumulation of ADM in A549/R cell line and derease the medium inhibition concentration(IC50).The overexpression of MRP mRNA was observed in A549/R cell line and this was significantly suppressed in As2O3-treated A549/R cells. Conclusion As2O3 can partically reverse the resistance to ADM in A549/R cell line and this effect may be related to the variation of MRP gene expression.