| β神经生长因子对内皮祖细胞一氧化氮合酶的影响 |
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| 引用本文： | 王中京,丁胜,赵湜,毛红,孙敏娴,王利.β神经生长因子对内皮祖细胞一氧化氮合酶的影响[J].武汉大学学报(医学版),2015,36(1):29-31,109. |
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| 作者姓名： | 王中京 丁胜 赵湜 毛红 孙敏娴 王利 |
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| 作者单位： | 华中科技大学同济医学院附属武汉市中心医院 |
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| 基金项目： | 武汉市卫生局资助项目(编号:WX11B06) |
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| 摘 要： | 目的:探讨β-神经生长因子(β-NGF)对大鼠内皮祖细胞(EPCs)内皮型一氧化氮合酶(eNOS)及一氧化氮(NO)合成的影响。方法:将β-NGF重组腺病毒(Ad-β-NGF)及其阴性对照感染至大鼠内皮祖细胞,同时设置空白对照,分别于24h、48h及72h进行检测。用qPCR及Western blot分别在mRNA和蛋白水平检测不同组eNOS表达情况,硝酸还原酶法测定培养液中NO的水平。结果:Ad-NGF感染组eNOS表达及NO合成明显高于对照组,差异有统计学意义(P<0.05),并随感染时间的延长而增加。结论:β-NGF能促进内皮祖细胞eNOS表达及NO合成,有利于内皮祖细胞发挥功能。
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| 关 键 词： | β-神经生长因子 内皮祖细胞 eNOS NO |
Influence of Beta-Nerve Growth Factor on the Production of eNOS and NO by Endothelial Progenitor Cells |
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| WANG Zhongjing;DING Sheng;ZHAO Shi;MAO Hong;SUN Minxian;WANG Li.Influence of Beta-Nerve Growth Factor on the Production of eNOS and NO by Endothelial Progenitor Cells[J].Medical Journal of Wuhan University,2015,36(1):29-31,109. |
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| Authors: | WANG Zhongjing;DING Sheng;ZHAO Shi;MAO Hong;SUN Minxian;WANG Li |
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| Institution: | WANG Zhongjing;DING Sheng;ZHAO Shi;MAO Hong;SUN Minxian;WANG Li;Dept.of Endocrinology,Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology; |
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| Abstract: | Objective:To evaluate the effect ofβ-nerve growth factor(NGF)on the production of endothelial nitric oxide synthase(eNOS)and NO by endothelial progenitor cells(EPCs).Methods:Primary rat EPCs were isolated from rat bone marrow.The purified EPCs were infected with AdEGFP-β-NGF and Ad-EGFP respectively,an addition pseudo-infection was acted as blank control.The effect was observed after infection at 24 h,48hand 72 h,respectively.The level of eNOS was tested by both q-PCR and Western-blot assay.The content of NO was determined by nitrate reductase.Results:The expression of eNOS and the production of NO increased greatly(P<0.05)compared with control group,which exhibited a time-dependent manner.Conclusion:β-NGF could improve the function of EPCs by promoting the expression of eNOS and NO. |
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| Keywords: | β-Nerve Growth Factor Endothelial Progenitor Cell Endothelial Nitric Oxide Synthase Nitric Oxide |
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