补阳还五汤联合运动训练对脑梗死大鼠神经元突触重建的影响

目的 :研究补阳还五汤联合运动训练对脑梗死大鼠神经元突触重建的影响。 方法 :用自体血栓经导管注入右侧大脑中动脉建立大鼠局灶性脑梗死模型,分别给予生理盐水、运动训练、补阳还五汤以及补阳还五汤联合运动训练处理,观察神经功能损伤情况,脑组织中突触体素P38(P38) 和生长相关蛋白-43(GAP-43)的动态表达变化。 结果 :运动训练、补阳还五汤和联合治疗均能够使大鼠神经功能损伤评分降低,P38,GAP-43表达增加,以联合治疗组最为明显。 结论 :补阳还五汤联合运动训练可改善脑缺血后神经行为症状,促进神经细胞突触再生重建,两者联合具有协同增效的作用。

Effect of Buyang Huanwu Decoction Combined with Sports Training on Neurons Synapse Remodeling in Rats with Focal Cerebral Infarction

Objective: To investigate the effect of Buyang Huanwu Decoction combined with sports training on the behavior and neurons synapse remodeling in rats with focal cerebral infarction. Method: Rats were subjected to embolic focal ischemia using homologous clots and treated with: saline, sports training, Buyang Huanwu Decoction, Buyang Huanwu Decoction combined with sports training, the expression of P38 and growth associated protein-43(GAP-43) were detected by immunohistochemical approach at 7th, 14th and 21st day after each management. Result: The sports training group, Buyang Huanwu group and the combined group all can reduce the damage score of rats nerve function, and the recovery was most significant in the combined group.The expression of P38 and GAP-43 in the combined group was remarkably increased compared with the sports training group and the Buyang Huanwu group. Conclusion: Buyang Huanwu Decoction combined with sports training can improve the scores of cerebral ischemia nerve behavior and promote synapse building and new contact of synapse regeneration,the combination of the decoction and sports showed excellent performances and synergism.