| RP-HPLC测定石蒜中加兰他敏含量 |
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| 引用本文： | 吴芳丽,李爱珍,毛慧芳.RP-HPLC测定石蒜中加兰他敏含量[J].中国中药杂志,2005,30(7):523-525. |
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| 作者姓名： | 吴芳丽 李爱珍 毛慧芳 |
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| 作者单位： | 浙江一新制药股份有限公司,浙江,兰溪,321100 |
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| 摘 要： | 目的:建立测定石蒜中加兰他敏含量的HPLC方法。方法:采用ODS色谱柱(46mm×150mm,5μm),以磷酸盐缓冲液(pH3～4)甲醇(93∶7)为流动相,流速10mL·min-1,二极管阵列检测器于289nm测定。结果:加兰他敏在3～30μg·mL-1线性关系良好(r=0.9997),最低检测限为0.30ng,测定加兰他敏的平均回收率为99.5%,RSD0.5%。结论:方法操作简便,结果可靠,重复性好,专属性强,将为石蒜的质量控制提供科学依据。
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| 关 键 词： | 高效液相色谱法 加兰他敏 石蒜 |
| 文章编号： | 1001-5302(2005)07-0523-03 |
| 收稿时间： | 2003/11/10 0:00:00 |
Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC |
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| WU Fang-li;LI Ai-zhen;MAO Hui-fang.Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC[J].China Journal of Chinese Materia Medica,2005,30(7):523-525. |
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| Authors: | WU Fang-li;LI Ai-zhen;MAO Hui-fang |
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| Institution: | Zhejiang Yixin Pharmaceutical Co. LTD, Lanxi 321100, China. |
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| Abstract: | OBJECTIVE: To establish a method for determination of galanthamine in Lycoris radiata. METHOD: HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm. RESULT: Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%). CONCLUSION: The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata. |
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| Keywords: | HPLC galanthamine Lycoris radiata |
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