Ultrastructural and physiological effects of the ionophore A23187 at identified frog neuromuscular junctions

The physiological and morphological effects of the calcium ionophore A23187 (calimycin) at the frog neuromuscular junction in vitro were examined. Miniature endplate potentials were recorded intracellularly during exposure to the ionophore. Preparations fixed 15 or 30 min after adding the drug to the incubating medium, which exhibited a greatly increased miniature endplate potential frequency, showed no obvious morphological differences when compared to controls with regard to synaptic vesicle number or distribution of vesicles within the terminal. However, after 45-60 min of exposure to the ionophore, when miniature endplate potential frequency had declined almost to zero, most of the nerve endings appeared devoid of synaptic vesicles and other organelles while the plasma membrane was intact. It is suggested that the apparent depletion of vesicles from the terminal induced by the calcium ionophore is a consequence of irreversible changes at the terminal.