p16基因蛋白在宫颈癌中表达的研究

目的　探讨抑癌基因p16与宫颈癌的关系。方法　采用S P免疫组化法检测正常宫颈组织 8例、宫颈炎组织 12例、宫颈鳞癌组织 10 0例中 p16基因蛋白的表达情况。 结果　p16基因蛋白在正常宫颈组织、宫颈炎组织、宫颈鳞癌组织阳性表达率及表达强度依次降低 87 5 %、66 67%、3 9 0 0 % ,3者有显著性差异 (P <0 0 5 ) ,p16蛋白在宫颈鳞癌中的表达随临床分期级别的增高而降低 :0期 75 0 0 %、Ⅰ期 62 5 0 %、Ⅱ期 48 72 %、Ⅲ期 17 0 7% ,4者差异显著 (P <0 0 5 ) ;随分化程度的降低而降低 :高分化 62 96%、中分化 3 6 5 9%、低分化 2 1 88% ,3者有显著差异 (P <0 0 5 )。p16蛋白在有淋巴结转移的宫颈癌中的表达为 2 1 43 % ,显著低于无淋巴结转移者 5 1 72 % ,2者有显著性差异 (P <0 0 5 )。结论　抑癌基因p16蛋白缺失与宫颈癌的发生、自然病程、恶性程度及淋巴转移有关。

THE EXPRESSION OF p16 IN CERI CAL CARCINOMA

Objective To discuss the relationship between tumor suppre ss or gene p16 protein and cervical carcinoma. Methods S-P immu nohistochemical method was used to measure the expression of p16 protein in 8 no rmal cervix uteri tissue. 12 cervictis tissue. 100 cervical squamous carcinoma t issue. Results The results showed the positive expression rat e of p16 protein in normal cervix uteri tissue, cervictis tissue and cervical sq uamous carcinoma in 87.50%, 66.67%, 39.00% respectiyely. They had apparent di fference (P<0.05). the expression of p16 protein, in cervical carcinoma wh ich decreased with the increase of clinical stage: 0 stage 50%, Ⅰ stage 62.50% , Ⅱ stage 48.72%, Ⅲ stage 17.07%. They had difference apparently (P<0. 05), and it decreased with the decrcase of differentiation 36.59% and poor diff erentiation 21.88%. They also had differtence(P<0.05). Its positive expre ssion had obvious difference in clinical stage and pathological grade of cervica l carcinoma (P<0.05), It also had difference apparently in lymph node meta stasis (P<0.05). Conclusions Tumor suppressor sene p16 protein loss with cervical carcinogenesis. natural course. malignancy and metast asis.