| 阿糖胞苷减少白血病细胞亮氨酸氨基肽酶的表达 |
| |
| 引用本文： | 马颖,李有杰,岳真,谢书阳. 阿糖胞苷减少白血病细胞亮氨酸氨基肽酶的表达[J]. 滨州医学院学报, 2010, 33(3): 164-166. DOI: 10.3969/j.issn.1001-9510.2010.03.002 |
| |
| 作者姓名： | 马颖 李有杰 岳真 谢书阳 |
| |
| 作者单位： | 滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003 |
| |
| 基金项目： | 山东省科技厅，滨州医学院科技计划项目 |
| |
| 摘 要： | 目的研究化疗药物阿糖胞苷对白血病细胞K562细胞亮氨酸氨基肽酶(leucine aminopeptidase,LAP)表达的影响,探讨其抗肿瘤机制。方法阿糖胞苷(终浓度442μmol/L)处理细胞48 h,倒置显微镜下观察细胞形态改变及细胞凋亡数目,RT-PCR方法检测LAP基因mRNA的表达。结果阿糖胞苷处理细胞48 h后,倒置显微镜下观察到细胞形态发生明显改变,凋亡细胞数显著增多;LAP基因mRNA的表达(0.429±0.061)明显低于对照组(0.789±0.059)(P<0.05)。结论减少白血病细胞株LAP的表达可能是阿糖胞苷致白血病细胞凋亡的机制之一。
|
| 关 键 词： | 亮氨酸氨基肽酶 阿糖胞苷 K562细胞 基因表达 |
Expression of leucine aminopeptidase reduced in K562 cells after cytosine arabinoside treatment |
| |
| MA Ying,LI Youjie,YUE Zhen,XIE Shuyang. Expression of leucine aminopeptidase reduced in K562 cells after cytosine arabinoside treatment[J]. Journal of Binzhou Medical College, 2010, 33(3): 164-166. DOI: 10.3969/j.issn.1001-9510.2010.03.002 |
| |
| Authors: | MA Ying LI Youjie YUE Zhen XIE Shuyang |
| |
| Affiliation: | MA Ying LI Youjie YUE Zhen XIE Shuyang Department of Biochemistry , Molecular Biology,Binzhou Medical University,Yantai 264003 |
| |
| Abstract: | Objective To investigate the mechanism of cytosine arabinoside inducing tomour cell apoptosis by suppressing the expression of leucine aminopeptidase(LAP).Methods 48 hours after K562 cells treated with cytosine arabinoside,the apoptotic cells were observed by inverted microscope and the expression of LAP mRNA was detected by RT-PCR.Results After treatment with cytosine arabinoside for 48 hours,the morphologic changes of cells were notable and the expression of LAP mRNA was decreased significantly.Conclusion... |
| |
| Keywords: | leucine aminopeptidase cytosine arabinoside K562 cells gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
