多效生长因子及血小板源性生长因子在增龄鼠椎间盘中的表达*

背景：在组织缺氧状态下，多效生长因子能够直接参与新生血管的生成，在人类腰椎间盘退变方面起重要的作用。 目的：观察增龄鼠椎间盘中多效生长因子和血小板源性生长因子的表达，及其与椎间盘退变的关系。 方法：取1，3，6，12，18月龄Wistar大鼠各10只。采用免疫组织化学染色方法检测椎间盘组织中多效生长因子及血小板源性生长因子的表达情况。采用Spearman相关分析方法对多效生长因子和血小板源性生长因子的相关性进行分析。 结果与结论：随着大鼠月龄的增加，椎间盘细胞中多效生长因子及血小板源性生长因子的表达有逐渐减低的趋势，但至18月龄又有所增加。同月龄组椎间盘细胞中，多效生长因子及血小板源性生长因子在终板的表达高于髓核和纤维环(P < 0.05)。多效生长因子及血小板源性生长因子在椎间盘组织中的表达有一定的相关性(P < 0.01)。提示大鼠椎间盘结构随月龄增加发生退行性变，多效生长因子及血小板源性生长因子参与了大鼠椎间盘的退变。

Expression of pleiotrophin and platelet derived growth factors in rat discs with increasing age

BACKGROUND: Pleiotrophin (PTN) directly participates in angiogenesis and plays an important role in humen degeneration of intervertebral disc under hypoxia condition. OBJECTIVE: To investigate the expression of PTN and platelet derived growth factor (PDGF) in rat discs at different months, so as to probe into the association of PTN and PDGF with degeneration of intervertebral disc. METHODS: Fifty Wistar rats were divided into 5 groups according to their different months: 1-, 3-, 6-, 12 and 18-month groups. The expression of PTN and PDGF in intervertebral disc was observed by SABC immunohistochemistry. The correlation between PTN and PDGF expression was analyzed by Spearman. RESULTS AND CONCLUSION: The expression of PTN and PDGF in intervertebral disc decreased as rat age (1-12 months) increasing, but it increased when it came to 18-month. In the same group, the expression of PTN and PDGF was higher in cartilage end plate than in annulus fibrosus and nucleus pulposus (P < 0.05). A high correlation was presented in the expression of PTN and PDGF (P < 0.01). The intervertebral disc of rat degenerates along with their ages of months. PTN and PDGF participate in intervertebral disc degeneration.