安徽省肠出血性大肠杆菌O157:H7宿主动物监测研究

目的了解安徽省肠出血性大肠杆菌O157:H7在宿主动物中的分布及其毒力基因携带状况,为控制O157:H7感染提供依据。方法用免疫磁珠分离法(IMS)对安徽省3个监测点的宿主动物粪便标本进行0157:H7的分离培养,并用多重引物PCR方法(MPCR)检测分离菌株的毒力基因。结果采集宿主动物粪便标本5 560份,检出O157:H737株,阳性率为0.7%,其中牛的阳性率最高,为1.1%。106株O157:H7菌株经过毒力基因测定,65.1%的菌株携带SLT、hly基因。O157:H7感染性腹泻的发病与家禽家畜的带菌正相关(R=0.87,P=0.01)。结论安徽省宿主动物中能检出肠出血性大肠杆菌O157:H7,且菌株携带毒力基因。提示要加强对宿主动物进行O157:H7的流行病学调查和监测工作。

Analysis of Surveillance Data of Escherichia coli O_(157):H_7 in Reservior from 2000 to 2005 in Anhui Province

Objective To understand the distribution of Escherichia coli O_(157):H_7 and its virulence genes isolated from various reservoir in Anhui Province.Methods O_(157):H_7 from the dejecta of the reservoirs in three surveillance sites was isolated and cultured by IMS.The virulence genes were isolated and detected with multiplex PCR assay.Results 37O_(157):H_7 samples were isolateded from 5560 animal samples,the total positive rate was 0.7%.There were differences about the positive rate among animals.The highest one was 1.1% in cows.65.1% of 106 O_(157):H_7 strains carried SLT and hly genes.The infectious diarrhea of E.coli O_(157):H_7 associated with the carrying status of E.coli O_(157):H_7 in live stock and poultry(R=0.87,P=0.01).Conclusions Escherichia coli O_(157):H_7 carrying virulence genes were popular in reservoirs in Anhui Province.It suggests that the epidemiological investigation and checking work for O_(157):H_7 should be reinforced.