| 丙戊酸钠对视网膜色素变性小鼠光感受器细胞的保护作用 |
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| 引用本文： | 彭媛,杨柳. 丙戊酸钠对视网膜色素变性小鼠光感受器细胞的保护作用[J]. 标记免疫分析与临床, 2009, 16(3): 156-159 |
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| 作者姓名： | 彭媛 杨柳 |
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| 作者单位： | 北京大学第一医院眼科,北京,100034;北京大学第一医院眼科,北京,100034 |
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| 摘 要： | 观察在视网膜变性小鼠（retinal degeneration mouse,rd小鼠）视网膜色素变性过程中,丙戊酸钠（valproate sodium,VPA）是否对光感受细胞具有保护作用。取48只新生rd小鼠,随机分为VPA组和正常对照组,每组24只。（1）VPA组,出生后每日以VPA100mg/kg的剂量进行腹腔注射;（2）对照组,出生后每日腹腔注射与VPA相同体积的生理盐水。于出生后7天、14天、21天、28天分别将VPA组和对照组的6只小鼠处死,取眼球做冰冻切片,行HE染色,对两组视网膜光感受器细胞数进行计数,并测量外核层厚度;用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（terminal deoxy-nucleotidyl transferase-mediated dUTP nick-end labeling,TUNEL法）检测凋亡细胞数,并对凋亡细胞数目进行统计分析。结果显示：（1）出生后7天VPA组光感受器细胞数和外核层厚度与对照组相比并无统计学差异,出生后14天、21天、28天,VPA组光感受器细胞和外核层厚度多于对照组,两者具有统计学差异（P〈0.05）;（2）两组光感受器细胞均于出生后7天开始出现凋亡,在出生后7天、14天VPA组外核层凋亡数少于对照组,两者具有统计学差异。提示VPA具有对抗视网膜色素变性小鼠光感受器细胞凋亡的作用,能延缓光感受器细胞的进行性退变。
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| 关 键 词： | 丙戊酸钠 视网膜色素变性 光感受器细胞 凋亡 神经保护 |
Neuroprotective Effect of Sodium Valproate on Photoreceptor Cell in rd Mice |
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| PENG Yuan,YANG Liu. Neuroprotective Effect of Sodium Valproate on Photoreceptor Cell in rd Mice[J]. Labeled Immunoassays and Clinical Medicine, 2009, 16(3): 156-159 |
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| Authors: | PENG Yuan YANG Liu |
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| Affiliation: | PENG Yuan,YANG Liu(Department of Ophthalmology,Peking University First Hospital,Beijing,100034,China) |
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| Abstract: | To explore whether sodium valproate(VPA) can protects photoreceptor cells on a mouse model of retinitis pigmentosa,48 rd mice were divided into VPA group and Normal Control group at random,24 in each group.VPA group received intraperitoneal injection of VPA 100mg/kg(10ml/kg) while the control group received saline 10ml/kg every day from the postnatal day 1(P1).Six mice were sacrificed at P7,P14,P21 and P28 respectively.Eyeballs were enucleated,and cut into frozen section and measured the number of photorece... |
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| Keywords: | Sodium valproic acid Retinitis pigmentosa Photoreceptorcells Apoptosis Neuroprotection |
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