| 人胚胎甲状腺褪黑素受体mRNA的检测 |
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| 引用本文： | 孙中安,黄超.人胚胎甲状腺褪黑素受体mRNA的检测[J].第二军医大学学报,2000,21(11):1062-1064. |
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| 作者姓名： | 孙中安 黄超 |
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| 作者单位： | [1]第二军医大学长征医院内分泌科,上海 [2]实验诊断科,上海 |
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| 摘 要： | 目的：检测胚胎甲状腺中是否表达褪黑素受体。方法：取人胚胎甲状腺，抽提总ＲＮＡ并用ＲＴ－ＰＣＲ方法检测其ｍＲＮＡ，阳性产物用自动测序仪测序。结果：人胚胎甲状腺总ＲＮＡ以ＭＴ１及ＭＴ２两种引物进行的ＲＴ－ＰＣＲ产物电泳呈现阳性条带，测序结果显示扩增产物与人类褪黑素受体ｃＤＮＡ相吻合。结论：人胚胎甲状腺组织中存在有褪黑素ＭＴ１，ＭＴ２两种受体的ｍＲＮＡ，表明该组织中有褪黑素ＭＴ１，ＭＴ２受体的表达。
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| 关 键 词： | 受体 褪黑素 逆转录聚合酶链反应 甲状腺 mRNA |
| 文章编号： | 0258-879X(2000)11-1062-03 |
| 修稿时间： | 2000年6月6日 |
Detection of melatonin receptor mRNA in thyroid of human embryos |
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| SUN Zhong An ,LIU Zhi Min ,HUANG Chao.Detection of melatonin receptor mRNA in thyroid of human embryos[J].Academic Journal of Second Military Medical University,2000,21(11):1062-1064. |
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| Authors: | SUN Zhong An LIU Zhi Min HUANG Chao |
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| Institution: | SUN Zhong An 1,LIU Zhi Min 1,HUANG Chao 2 |
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| Abstract: | Objective: To make it clear whether the thyroid in human embryo express melatonin(MEL) receptor or not. Methods: Thyroids was collected to obtain total RNA and the mRNA of melatonin MT1 or MT2 was detected by RT PCR method. The positive production was sequenced with auto sequencing instrument. Results: The electrophoretic results of RT PCR product by MT1 and MT2 primer were all positive. Sequencing results indicated that the positive product coincided with cDNA of human melatonin receptor. Conclusion: There exists melatonin receptor mRNA in human embryo thyroid, either MT1 and MT2, which shows human embryo thyroid express both MT1 or MT2 receptor. |
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