成骨生长肽对大鼠骨髓基质细胞增殖和成骨分化的作用

目的：观察成骨生长肽诱导体外培养的大鼠骨髓基质细胞增殖及向成骨分化的作用。方法：取6周龄SD大鼠，贴壁法分离培养骨髓基质细胞，在不同浓度成骨生长肽的诱导下，观察细胞形态变化，绘制骨髓基质细胞生长曲线，碱性磷酸酶和钙结节组织化学染色。结果：成骨生长肽对骨髓基质细胞增殖和成骨分化的作用呈剂量依赖性：成骨生长肽浓度在10^-10及10^-11mol／L时促进骨髓基质细胞增殖，而在10^-8及10^-9mol／L时对骨髓基质细胞的增殖略有抑制作用；成骨生长肽浓度在10^-10及10^-11mol／L时骨髓基质细胞碱性磷酸酶染色基本呈阴性，与对照组相比差异无统计学意义，而在10^-8及10^-9mol／L浓度下可以显著提高骨髓基质细胞碱性磷酸酶染色的阳性率。结论：成骨生长肽可以明显促进大鼠骨髓基质细胞增殖及向成骨细胞分化，其促成骨活性具有显著的浓度依赖性。

Effects of osteogenic growth peptide to the proliferation and differentiation of bone marrow stromal cells

Objective: To investigate the effects of osteogenic growth peptide(OGP) to the proliferation and differentiation of cultured bone marrow stromal cells (BMSCs) of rats. Methods: The SD rats(age 6 weeks) were sacrified,and the bone marrow stromal cells as the adherent stromal cell population were separated from the bone marrow culcure. The proliferation curves of bone marrow stromal cells which were conditioned cultured with four kind of different concentrations of osteogenic growth peptide were measured with the MTT method,and the osteogenesis markers including alkaline phosphatase and calcic deposition detected by histochemical staining. Results: Osteogenic growth peptide at the concentration of 10-10,10-11 mol/L promoted the proliferation of bone marrow stromal cells,while at the concentrations of 10-8,10-9 mol/L suppressed the proliferation of bone marrow stromal cells. However,osteogenic growth peptide at the concentration of 10-9,10-8 mol/L advanced the ratio of positive cells in alkaline phosphatase histochemical staining. Conclusion: Osteogenic growth peptide shows distinct effects on the proliferation and differentiation of bone marrow stromal cells depending on its concentration. Osteogenic growth peptide at the concentration of 10-8,10-9 mol/L can promote bone marrow stromal cell differentiation to the osteogenic in vitro.