原癌基因c-fos在大鼠急性局灶性脑缺血再灌注损伤和高压氧治疗后表达的变化

目的　探讨原癌基因 c- fos在大鼠急性局灶性脑缺血 /再灌注损伤和高压氧 (HBO)治疗后表达的变化。方法　用血管内细丝栓堵大鼠的大脑中动脉 (MCA ) ,制作成局灶性脑缺血动物模型 ,利用免疫组织化学方法 ,观察了 MCA缺血 1 h,再灌注 4、1 1、2 3、71 h c- fos癌基因表达的变化 ,在以上期间同时应用常压 0 .1 MPa纯氧或 0 .2 5 MPa HBO于开始缺血后 2、9、2 1、45和 6 9h分别治疗 1次 (1 h)。结果　缺血后 5 h,c- fos原癌基因在梗塞区皮层、纹状体、视前区均有明显表达 ,持续 1 2～ 2 4h后开始减弱 ,72 h基本消失。 HBO治疗后 ,各时间点 c- fos癌基因在上述区域的表达均明显减弱。结论　 HBO可明显抑制大鼠急性局灶性脑缺血 /再灌注损伤区 c- fos癌基因的表达

Expression of c fos in rat brain following acute focal cerebral ischemia reperfusion and hyperbaric oxygen therapy

Objective To investigate the expression of c fos in rat brain following acute focal cerebral ischemia reperfusion (IR) and hyperbaric oxygen(HBO)therapy.Methods Rat model of focal cerebral ischemia induced by intraluminal filament occlusion to the middle cerebral artery(MCA) was used.The expressions of c fos in rat brain were examined after 1 h of ischemia with 4,11,23 and 71 h of reperfusion(removal of MCA occlusion) respectively using the immunohistochemistry method.At the same time,0.1 MPa 100% O-2 or 0.25 MPa HBO treatment for 1 h each time was applied at 2,9,21,45 and 69 h after the begining of ischemia.Results FOS protein was strongly expressed in cortex,striatum and preoptic area after 5 h of IR,which lasted 12~24 h and then disappeared in 72 h.With HBO treatment,the FOS protein was less expressed in these above brain areas at every observing time points.Conclusions HBO treatment could obviously inhibit the expression of c fos in rat brain following acute focal cerebral ischemia reperfusion injury.