| 提高石蜡包埋组织中提取DNA质量的实验研究 |
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| 引用本文： | 盖宝东,房学东,金仲田,张德恒,郑泽霖. 提高石蜡包埋组织中提取DNA质量的实验研究[J]. 吉林大学学报(医学版), 2003, 29(1): 115-118 |
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| 作者姓名： | 盖宝东 房学东 金仲田 张德恒 郑泽霖 |
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| 作者单位： | 1. 吉林大学中日联谊医院基本外科,吉林,长春,130031
2. 日本东北大学医学部先进外科 |
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| 基金项目： | 中-日合作项目;1993; |
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| 摘 要： | 目的 :确定从石蜡包埋组织中提取 DNA的最佳条件。方法 :改变石蜡切片厚度、组织脱蜡时间、消化液蛋白酶 K浓度、组织消化时间等参数 ,组合出 96种不同提取 DNA的条件 ,比较各种条件下所提取 DNA的数量和质量。结果 :各种条件均可以提取出一定数量的 DNA,但有些条件所提取的 DNA不适合于做 PCR及 DNA序列测定。结论 :从石蜡包埋组织中提取 DNA的最佳条件为 ,1 0 .0 μm组织切片 ;切片脱蜡 2次 ,脱蜡时间分别为 2 h和 1 2 h;消化液中蛋白酶 K浓度5 0 0 mg· L-1;组织消化 1 2 h
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| 关 键 词： | 石蜡包埋 DNA/分离和提纯 聚合酶链反应 序列分析,DNA |
| 文章编号： | 1671-587X(2003)01-0115-04 |
| 修稿时间： | 2002-05-24 |
Study on Improving the Quantity and the Quality of DNA Extraction from Paraffin-embedded Tissues |
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| GAI Bao dong ,FANG Xue dong ,JIN Zhong tian ,ZHANG De heng ,ZHENG Ze lin. Study on Improving the Quantity and the Quality of DNA Extraction from Paraffin-embedded Tissues[J]. Journal of Jilin University: Med Ed, 2003, 29(1): 115-118 |
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| Authors: | GAI Bao dong FANG Xue dong JIN Zhong tian ZHANG De heng ZHENG Ze lin |
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| Affiliation: | GAI Bao dong 1,FANG Xue dong 1,JIN Zhong tian 2,ZHANG De heng 1,ZHENG Ze lin 1 |
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| Abstract: | Objective:To confirm the best condition of DNA extraction from paraffin embedded tissues. Methods:Compared the quantity and the quality of DNAs which are extracted from paraffin embadded tissues though different conditions, in which different thickness of the slice,different time of dewaxing,different concentr ation of proteinase K and different time of digestion. Results:All conditions can extract a certain quantity of DNA,but some DNAs are unfit for PCR and sequencing. Conclusions:The best condition of DNA extraction from paraffin embedded tissues is obvious, 10.0 μm slice, separate 2 and 12 hours dewax time,500 mg·L -1 proteinase K and 12 hours digestion. |
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| Keywords: | paraffin embadded tissues DNA/isolation and purification polymerase chain reaction sequence analysis DNA |
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