幼兔心肌缺血再灌注损伤中生化指标变化的研究

目的 观察幼兔未成熟心肌缺血再灌注损伤后生化指标的变化及异丙酚对损伤的影响.方法 取日本大耳幼兔24只(日龄21-28d)随机分为3组(n=8):缺血再灌注组(A组)、异丙酚预处理组(B组)和对照组(C组).A、B组以结扎冠状动脉前降支制作在体兔心肌缺血再灌注损伤动物模型,B组缺血前给予异丙酚;再灌注120 min时抽动脉血测血清一氧化氮(NO)浓度、一氧化氮合酶(NOS)活性、丙二醛(MDA)浓度、超氧化物歧化酶(SOD)活性和同型半胱氨酸(Hcy)浓度.结果 与C组比较,A组血清MDA及Hcy浓度显著增高(P＜0.05),NOS活性显著降低(P＜0.05),SOD活性降低但无显著性意义;B组血清SOD活性、NO浓度升高(P＜0.05);与A组比较,B组血清NO浓度、SOD及NOS活性增高,MDA、Hcy浓度下降(P＜0.05).结论 异丙酚对实验性幼兔心肌缺血再灌注损伤有保护作用,可能与异丙酚抗氧化作用、激活NOS、增加NO浓度以及减轻Hcy堆积等机制有关.

Investigation the changes of biochemical markers in immature rabbit undergoing myocardial ischemical reperfusion injury

Objective To investigate the changes of biochemical markers in rabbit immature myocardium which have underwent MIRI and the cardioprotective effects of Propofol.Methods Twenty-four rabbits either sexes aged 21-28 days were randomly divided into three groups(n=8 each):Ischemia-reperfusion group(A group) Propofol group(B group) and control group(C group).In three groups,left common carotid artery was cannulated for blood collection.Chest was opened and myocardial ischemia was induced by occlusion of anterior descending branch of left coronary artery and confirmed by cyanosis of local myocardial and by elevation or depression of S-T segment and /or high T wave.Blood samples were taken for detection of plasma SOD,NOS activity and plasma NO,MDA,Hcy concentration.Results To compare with group C,the plasma Hcy and MDA concentration were significantly higher(P<0.05) and NOS activity was siginificantly lower in group A.In group B,the plasma Hcy and MDA concentration were significantly lower than those in A group(P<0.05)and the plasma SOD,NOS activity and NO concentration were significantly higher than those in A group(P<0.05).Conclusion Propofol has cardioprotective effects on immature MIRI and the mechanism of protection is related to anti-oxidation,promotion of NOS activity and reducing the concentration of Hcy.