精浆抗精子抗体对精子形态及运动特征的影响

目的:探讨精浆抗精子抗体(AsAb)对精子形态和运动特征的影响。方法:检测251例男性不育患者精液样本,采用酶联免疫法进行AsAb检测,计算机自动分析精子运动特征,采用精子形态检测系统下人工修正方法进行精子形态分析。结果:251例男性不育患者中,AsAb阳性率为17·13%。AsAb阳性组正常形态精子百分率与阴性组无显著性差异(P>0·05),大头精子和锥形头精子百分率显著高于AsAb阴性组(P<0·05,P<0·005)。精浆AsAb阳性组a+b级精子百分率,精子运动参数MAD、LIN、WOB均明显低于阴性组,两组比较差异有显著性(P<0·05,P<0·01)。结论:精浆AsAb不影响正常形态精子百分率,但能使锥形头精子百分率显著升高;使a+b级精子百分率、精子运动参数MAD、LIN、WOB明显降低。

Effect of antisperm antibody of seminal plasma on sperm morphology and motion parameters

Objective:To study effect of AsAb of seminal plasma on sperm morphology and motion parameters.Methods:Sperm samples were obtained from 251 patients of male infertility. AsAb was detected by indirectly improved ELISA method. The sperm morphology and motion parameters analysis using semiautomatic sperm morphology analyzer was performed.Results:AsAb positive rate was 17.13% in 251 semen samples of patients. The percentage of normal morphology sperm of AsAb positive group was no statistics difference in comparison with AsAb negative group (P>0.05).But the percentages of large head sperm and tapered head sperm were markedly higher than that of AsAb negative group (P<0.05,P<0.005).The percentage of grade a+b sperm, MAD, LIN, WOB of sperm motion parameters were markedly lower than that of AsAb negative group (P<0.05).Conclusion:AsAb of seminal plasma don't influence the percentage of normal morphology sperm. But it can make the percentage of grade a+b sperm, MAD, LIN and WOB of sperm motion parameters descended.