| 靶向PEG10的siRNA真核表达载体对裸鼠肝癌移植瘤生长的影响 |
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| 引用本文： | 黄锦,唐滔,林菊生,董旭□.靶向PEG10的siRNA真核表达载体对裸鼠肝癌移植瘤生长的影响[J].中国现代医学杂志,2008,18(4). |
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| 作者姓名： | 黄锦 唐滔 林菊生 董旭□ |
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| 作者单位： | 1. 华中科技大学同济医院肝病研究所,湖北,武汉,430030
2. 华中科技大学同济医院普通外科,湖北,武汉,430030 |
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| 摘 要： | 目的探讨靶向PEG10的siRNA真核表达载体(psiRNA-PEG10)对裸鼠人肝癌移植瘤生长的影响。方法建立人肝癌细胞HepG2的荷瘤裸鼠模型,在接种后第14天分别将psiRNA空载体和psiR-NA-PEG10瘤内多点注射进行治疗,观察肿瘤大小变化、组织形态学及超微病理改变。结果psiRNA-PEG10治疗组肿瘤生长受到明显抑制,与生理盐水组和psiRNA组比较差异有显著性(P<0.001),抑瘤率明显高于其他两组,电镜下可见明显的细胞凋亡。结论靶向PEG10的siRNA真核表达载体对裸鼠肝癌移植瘤生长有抑制作用。
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| 关 键 词： | PEG10 siRNA 裸鼠 肝癌移植瘤 靶向 siRNA 真核表达载体 鼠肝癌 移植瘤生长 影响 nude mice human hepatocellular carcinoma transplanted growth gene targeting vectors eukaryotic expression 抑制作用 细胞凋亡 电镜 抑瘤率 比较差异 生理盐水 |
Influence of siRNA eukaryotic expression vectors targeting PEG10 gene on growth of transplanted human hepatocellular carcinoma in nude mice |
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| HUANG Jin,TANG Tao,LIN Ju-sheng,DONG Xu-yang.Influence of siRNA eukaryotic expression vectors targeting PEG10 gene on growth of transplanted human hepatocellular carcinoma in nude mice[J].China Journal of Modern Medicine,2008,18(4). |
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| Authors: | HUANG Jin TANG Tao LIN Ju-sheng DONG Xu-yang |
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| Institution: | HUANG Jin1,TANG Tao2,LIN Ju-sheng1,DONG Xu-yang1 (1.Institute of Liver Deseases,Affiliated Tongji Hospital of Huazhong University of Science , Technology,Wuhan,Hubei 430030,P.R.China,2.Department of General Surgery,P.R.China) |
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| Abstract: | Objective To investigate the influence of siRNA eukaryotic expression vectors targeting PEG10 gene on growth of human transplanted human hepatocellular carcinoma in nude mice. Methods HepG2 cell line was implanted subcutaneously into nude mice. Fourteen days after implantation,psiRNA-PEG10 and psiRNA were injected into nude mice,respectively. The tumor volume was measured. The histopathological changes of the tumors were observed by HE staining and electron microscope. Results Growth of hepatocellular carci... |
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| Keywords: | PEG10 siRNA nude mice transplanted hepatocellular carcinoma |
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