| 大鼠角膜碱烧伤后ILK和VEGF的表达与角膜新生血管的相关性研究 |
| |
| 引用本文： | 戴鹏飞,王峰,郑玉萍,权彦龙,张菁菁.大鼠角膜碱烧伤后ILK和VEGF的表达与角膜新生血管的相关性研究[J].国际眼科杂志,2012,12(2):208-211. |
| |
| 作者姓名： | 戴鹏飞 王峰 郑玉萍 权彦龙 张菁菁 |
| |
| 作者单位： | 西安交通大学医学院第二附属医院眼科,中国陕西省西安市,710004 |
| |
| 基金项目： | 中国国家自然科学基金资助项目(No.30772598) |
| |
| 摘 要： | 目的:研究大鼠角膜碱烧伤后整合素连接激酶(ILK)、血管内皮生长因子(VEGF)的表达及其与角膜新生血管(CNV)的相关性.方法:通过碱烧伤Sprague-Dawley(SD)大鼠角膜制备角膜新生血管动物模型,采用形态学方法观察碱烧伤后新生血管的生成,免疫组织化学方法检测ILK及VEGF在碱烧伤后不同时间点的表达情况.结果:ILK及VEGF在正常大鼠角膜上皮中仅有微弱表达,而在碱烧伤后各时间点的角膜上皮以及血管内皮中均有明显表达(P<0.01).碱烧伤后3~5d,可见二者阳性表达,且迅速增加;7~10d,二者表达达到高峰;14d以后二者表达逐渐减弱,与形态学观察CNV生长过程一致.即碱烧伤后3~5d新生血管生长迅速,7~10d生长达到高峰,14d以后血管开始逐渐减少退化.ILK及VEGF表达的变化均与CNV的变化呈正相关(ILK与CNV面积相关系数r=0.900,P<0.01;VEGF与CNV面积相关系数r=0.878,P<0.01),且二者之间也存在正相关性(r=0.926,P<0.01).结论:大鼠角膜碱烧伤后ILK及VEGF均有明显表达,二者作为重要的调控子共同参与了CNV的形成过程.
|
| 关 键 词： | 整合素连接激酶 血管内皮生长因子 角膜新生血管 碱烧伤 免疫组织化学 |
| 收稿时间： | 2011/12/16 0:00:00 |
| 修稿时间： | 1/4/2012 12:00:00 AM |
Relationship between corneal neovascu- larization and the expression of ILK and VEGF in cornea of rats after alkaline burn |
| |
| Peng-Fei Dai,Feng Wang,Yu-Ping Zheng,Yan-Long Quan and Jing-Jing Zhang.Relationship between corneal neovascu- larization and the expression of ILK and VEGF in cornea of rats after alkaline burn[J].International Journal of Ophthalmology,2012,12(2):208-211. |
| |
| Authors: | Peng-Fei Dai Feng Wang Yu-Ping Zheng Yan-Long Quan and Jing-Jing Zhang |
| |
| Institution: | Department of Ophthalmology, the Second Hospital of Medical College of Xi-an Jiaotong University, Xi-an 710004, Shaanxi Province, China;Department of Ophthalmology, the Second Hospital of Medical College of Xi-an Jiaotong University, Xi-an 710004, Shaanxi Province, China;Department of Ophthalmology, the Second Hospital of Medical College of Xi-an Jiaotong University, Xi-an 710004, Shaanxi Province, China;Department of Ophthalmology, the Second Hospital of Medical College of Xi-an Jiaotong University, Xi-an 710004, Shaanxi Province, China;Department of Ophthalmology, the Second Hospital of Medical College of Xi-an Jiaotong University, Xi-an 710004, Shaanxi Province, China |
| |
| Abstract: | AIM:To investigate the relationship between corneal neovascularization(CNV) and the expression of integrin-linked kinase(ILK) and vascular endothelial growth factor(VEGF) in cornea of rats after alkaline burn.METHODS:CNV model was established by alkaline cauterization of the central cornea of Sprague-Dawley(SD) rats.At the different time points,morphologic changes in CNV were observed with slit-lamp microscope,and the expression of ILK and VEGF in cornea was detected by immunohistochemical method and computer image analysis.RESULTS:ILK and VEGF were lowly expressed in epithelium of cornea in the control group,and strongly expressed in epithelium,endothelium and the endothelium cells of CNV in the experiment group(P<0.01).The expression of ILK and VEGF in cornea after alkaline burn,increased within the 3rd-5th day,reached the peak within the 7th-10th day,and decreased after the 14th day.The CNV in cornea after alkaline burn was found on the 3rd day,increased on the 5th day,reached the peak of area within the 7th-10th day,and decreased after the 14th day.The expression of ILK and VEGF showed positive correlation at different time points(r=0.926,P<0.01).And they were positively related to CNV(ILK and area of CNV,r=0.900,P<0.01;VEGF and area of CNV,r=0.878,P<0.01).CONCLUSION:ILK and VEGF were expressed significantly in cornea of rats after alkaline burn.And they involved in the regulation of CNV. |
| |
| Keywords: | integrin-linked kinase vascular endothelial growth factor corneal neovascularization alkaline burn immunohistochemistry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《国际眼科杂志》浏览原始摘要信息 |
| 点击此处可从《国际眼科杂志》下载免费的PDF全文 |
|
