茶多酚对体外培养鼠晶状体抗氧化损伤作用的研究

目的:观察茶多酚(tea polyphenols,TP)对氧化损伤鼠晶状体的形态学及抗氧化系统的变化,研究其对晶状体氧化损伤的保护作用.方法:采用体外培养鼠晶状体的氧化损伤模型,设置正常对照组、氧化损伤(H2O2)组和TP(H2O2+TP)组,分别于12,24,48h后观察各组晶状体的混浊情况,并检测各组晶状体的抗氧化酶系统中的超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性以及脂质过氧化反应终产物丙二醛(MDA)的含量.结果:正常对照组晶状体均保持透明,未见白内障形成;H2O2组大鼠离体晶状体随作用时间的延长,晶状体的混浊程度逐渐明显增加;TP组的晶状体混浊较H2O2组明显减轻,白内障形成不明显.晶状体混浊相对灰度值组间比较,差异有统计学意义(P<0.05).H2O2组大鼠晶状体组织中MDA 含量较对照组明显增高(P<0.05),而GSH-Px和SOD明显下降(P<0.05),TP组与H2O2组相比,其晶状体中MDA降低(P<0.05),但仍高于对照组,而GSH-Px和ATP含量明显升高,差异均有统计学意义(P<0.05).结论:TP可提高氧化损伤晶状体的抗氧化能力,降低脂质过氧化物水平,从而延缓白内障的发生和发展,为临床白内障的诊疗提供了新的理论基础.

Research of anti-oxidative damage with tea polyphenols on rat lens cultured in vitro

AIM:To observe tea polyphenols(TP) on the changes of the lens morphological and antioxidative system in rat of oxidative damage,and to research the protection of TP on oxidative damage to the lens.METHODS:In vitro oxidative damage model was established,the untreated group,oxidative damage group(H2O2) and experimental groups((H2O2+TP)were set respectively.After 6,12,24 and 48 hours,the cloudy conditions of lens were observed in every group,and the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) activity and malondaldehyde(MDA) content were detected of each lens.RESULTS:Untreated group’s lens all maintained transparent.Along with the extension of action time,cloudy degree of the lens gradually increased obviously in oxidative damage group.The lens cloudness in tea polyphenols group was less than that in oxidative damage groups.The differences among groups in lens opacity relative grey value were statistically significant(P<0.05).MDA content of lens tissue in oxidative damage group was significantly higher than that in control group(P<0.05),while GSH-Px and SOD decreased significantly(P<0.05).TP group being compared with oxidative damage group,its MDA in lens reduced(P<0.05),but still higher than that in control group,and GSH-Px,ATP levels increased obviously(P<0.05),all the differences were statistically significant.CONCLUSION:TP can improve the lens oxidation resistance to oxidative damage,reduce lipid peroxide level,thus delay the origin and development of cataract,and provide new theory basis for the clinical diagnosis and treatment of cataracts.