叶黄素对糖尿病大鼠肾损伤的缓解作用

目的研究叶黄素对糖尿病大鼠肾损伤的影响并探讨其可能机制。方法采用四氧嘧啶诱导大鼠糖尿病模型,灌胃给予叶黄素4w,测定大鼠血浆叶黄素水平、肾重、肾脏指数、BUN、SCr、CCr、24h尿蛋白及肾组织SOD、GSH-Px活性、GSH、MDA含量、TNF-α、IL-6水平。结果叶黄素5mg/kg.bw和20mg/kg.bw剂量组大鼠肾重、肾脏指数、BUN、SCr、CCr、24h尿蛋白均低于糖尿病大鼠组,差异有统计学意义(P<0.05);而肾组织SOD与GSH-Px活性、GSH含量高于糖尿病大鼠组(P<0.05),MDA含量低于糖尿病大鼠组(P<0.05),肾组织TNF-α、IL-6低于糖尿病大鼠组(P<0.05)。结论叶黄素能缓解糖尿病大鼠的肾功能损害,其机制可能与叶黄素升高抗氧化酶活性,降低肾组织的氧化应激水平以及减少促炎性细胞因子的表达有关。

EFFECT OF LUTEIN ON RENAL INJURY IN ALLOXAN INDUCED DIABETIC RATS

Objective To study the effect of lutein on diabetic nephropathy in alloxan-induced diabetic rats and its possible mechanism.Method The experimental diabetes in Sprague-Dawley rats was induced by alloxan and the rats were were given lutein intragastrically for 4 w,then the kidney weight,the ratio of kidney weight to body weight(KW/BW) ,blood urea nitrogen(BUN) ,serum creatinine(SCr) ,creatinine clearance rate(CCr) ,urinary protein,serum level of lutein,renal tissue superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities,glutathione(GSH) and malondialdehyde(MDA) levels and and renal tissue tumor necrotic factor α(TNF-α) ,interleukin 6(IL-6) levels were determined. Results Compared with the diabetic group,kidney weight,KW/BW,BUN,SCr,urinary protein were decreased in lutein group(P<0.05) ,the renal tissue SOD and GSH-Px activities,GSH were increased(P<0.05) and MDA level was decreased(P<0.05) in lutein group,and renal tissue TNF-α,IL-6 levels were decreased significantly in lutein group(P<0.05) Conclusion Oral administration of lutein can delay the renal injury of diabetic rats. The possible mechanism may be related with the effect of lutein in increasing activities of antioxidant enzymes and reducing lipid peroxidation and decreasing the cytokines in rat kidney.