宫颈病变中双链RNA依赖的蛋白激酶与HPV感染的相关性

目的：通过检测双链RNA依赖的蛋白激酶（PKR）、人乳头瘤病毒（HPV）在宫颈病变组织中的表达，探讨PICR在宫颈病变发生发展中的意义及可能机制。方法：选择临床病历资料和病理资料完整的宫颈癌患者共37例、同期宫颈上皮内瘤样病变（CIN）Ⅰ-Ⅲ患者56例为研究对象，因子宫肌瘤等良性病变切除子宫的正常宫颈组织30例为对照。采用免疫组织化学SABC法检测PICR及HPV16/18E6的表达。分析二者与宫颈病变发生发展的关系。结果：宫颈癌组织中PKR表达阳性率为54．1％，高于CINⅢ。CINⅠ-Ⅱ组和正常宫颈组，差异有统计学意义（P〈O．05）；在宫颈癌组中PICR表达与HPV16／18E6表达存在负相关（r=-0．554，P〈0．05）；在CINⅢ组中PKR表达与HPV16／18E6表达存在正相关（r=0．480，P=0．032）。结论：PKR的异常表达在CINⅢ向宫颈癌发展过程中可能发挥主要作用．此外SABC法测定PKR和E6的表达有可能成为筛选子宫颈癌的早期指标。

Relationship Between the Expression of PKR and High Risk HPV in Human Cervical Cancer and Pre-malignant Lesion

Objective： To study the expression of PKR, high risk HPV （HPV16/18） in cervical cancer and pre-malignant lesion, and discuss the significance and the possible mechanism in occurrence and development of cervical cancer and pre-malignant lesion by PKR. Methods： From February, 2006 to February, 2007, we took patients with cervical cancer （n=37） and patients with cervical intraepithelial neoplasia （CIN, n=56） treated in the department of gynecology in Tianjin first central hospital and Huanghe hospital, and compared with healthy donor （n=30）. In the 123 cases, we use immuno-histochemical SABC staining method to detect the expression of PKR and HPV16/18 E6 and analyze the happening and developing of cervical cancer and pre-malignant lesion and the effect in cervical lesion induced by PKR. Result： The positive rate of PKR expression in cervical cancer tissues was 54.1%. The positive rate of PKR expression in the cancer group is higher than CIN groups and the normal cervical group （P 〈0.05 ）. In the cancer group, the expression of PKR and HPV has inverse correlation （ r =-0.554, P 〈0.05 ）, and in the CIN Ⅲgroup, the expression of PKR and HPV has inverse correlation （r=0.480, P =0.032）. Conclusion： PKR was strongly associated with a decreased risk of developing cervical cancer, and HPV E6 gene has relation to modulate PKR expression. The expression of PKR and E6 could be an indicator in the prognosis of cervical cancer.