纳络酮对脑缺血-再灌注海马细胞凋亡的影响

目的观察缺血-再灌注期间海马细胞的凋亡及纳络酮对神经细胞的保护作用.方法20只新西兰大白兔随机分成 4 组(n=5)正常对照组、单纯缺血组(缺血组,夹闭颈总动脉和椎动脉30min)、缺血-再灌注组(再灌注组,夹闭颈总动脉和椎动脉30min后开放6h)、缺血-再灌注加纳络酮干预组(纳络酮组, 0.8 mg/kg静注及0.3mg/kg静滴).然后用流式细胞仪检测干预前、后的海马细胞的凋亡状况.结果缺血组(17.87%±3.04%)、再灌注组(38.84%±12.86%)的细胞凋亡比率显著高于对照组(5.94%±0.59%,P<0.01);再灌注组又明显高于缺血组 (38.84%±12.86% vs 17.87%±3.04% )和纳络酮组(38.84%±12.86% vs 8.96%±0.97%,P<0.01);而对照组与纳络酮组间则无显著差异.结论①兔全脑缺血后30min出现海马细胞凋亡;再灌注6h后,上述改变加重.②纳络酮对缺血-再灌注引起的神经细胞凋亡有一定的保护作用.

Nalxone decreases the ratio of apoptosis of the rabbit's hippocampus cells induced by ischemia and reperfusion

Objective:To study the protective effect of Nalonxe on the hippocampus cells of rabbits induced by ischemia and reperfusion. Methods:20 New Zealand white rabbits were randomly divided in four groups: control group, ischemia (bilateral common carotid artery and vertebral artery were occluded for 30mins) group, ischemia-reperfusion(above mentioned occluded artery were opened for 6hours) group, reperfusion associated with Naloxone therapy(0.8mg/kg iv and then 0.3mg/kg vd.) group. At the end of intervention, the ratio of apoptosis of hippocampus cells were assayed by flow cytometry. Results:1.The ratio of apoptosis of ischemia group and ischemia-reperfusion group were all significant higher than control group(P<0.01 respectively); and the ratio of ischemia-reperfusion group was significant higher than ischemia and naloxone group(P<0.01 respectively). (2. There) was no remarkable difference between that of naloxone therapy group and control group.Conclusion:Ischemia and ischemia-reperfusion may lead to apoptosis of hippocampus cells. Naloxone significantly reduces apoptosis of hippocampus cells and provide protective effect on hypoxia and reperfusion injure.