普罗布考与阿托伐他汀联合用药对高脂饮食自发性高血压大鼠血脂的影响

目的评价普罗布考和阿托伐他汀联合用药的调脂作用。方法将92只雄性自发性高血压大鼠随机分为普通饲料+生理盐水组(C组)、高脂饲料+生理盐水组(M组)、高脂饲料+普罗布考组(P组)、高脂饲料+阿托伐他汀组(A组)和高脂饲料+阿托伐他汀+普罗布考组(P+A组),实验8周后处死。实验前及实验结束时均抽血查血脂,包括总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C),以及肝功能,如谷丙转氨酶(GPT)和谷草转氨酶(GOT)。结果高脂饮食各组动物血清TC、LDL-C、HDL-C水平较实验前均明显升高(P<0.01),TG明显下降(P<0.01);各用药组动物(尤其是P+A组)TC、LDL-C均明显低于M组(P<0.01);P+A组血清HDL水平明显低于M组和A组(P<0.01),TG水平明显低于M组(P<0.01),并低于P组(P<0.05);A组TG水平亦明显低于M组(P<0.01)。高脂饮食各组动物血清GPT、GOT水平均较实验前明显升高(M组最高),且P+A组明显低于A组(均P<0.01)。结论高脂饮食可导致血脂紊乱及肝功能损害;普罗布考与阿托伐他汀联合用药可增强调脂作用,且较两者单独用药效果更佳;两者联合用药可减轻高脂饮食对肝功能的损害,并减轻阿托伐他汀的肝毒性。

Effects of Combination Therapy of Probucol and Atorvastatin on Adjusting Lipids in Spontaneously Hypertensive Rat with High Lipids Diet

Objective To evaluate the effects of combination therapy of probucol and atorvastatin on adjusting lipids.Methods 92 spontaneously hypertensive rats(SHR) were randomly divided into the group C(control group) fed with normal diet and normal saline(NS),and group M,group A,group P and group P+A all fed with high lipids diet.At the same time,group M was added with NS,group P with probucol(150 mg/kg/day),group A with atorvastatin(10 mg/kg/day),and group P+A with probucol(150 mg/kg/day) plus atorvastatin(10 mg/kg/day).After 8 weeks,all rats were killed.The total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),glutamic-pyruvic transaminase(GPT) and glutamic-oxal(o)acetic transaminase(GOT) were measured respectively before and after experiment.Results The serum levels of TC,LDL-C,HDL-C in every group with high lipids diet were obviously higher than baseline data(P<0.01),but TG level decreased.The serum levels of TC and LDL-C in the group P,group A and group P+A were significantly lower than that in the group M(P<0.01),especially in the group P+A.The serum level of HDL-C in the group P+A was obviously lower than that in the group M and group A(P<0.01).The serum levels of TG in the group P+A and group A were significantly lower than that in the group M(P<0.01),and that in the group P+A was lower than that in the group P(P<0.05).The serum levels of GPT and GOT in every group with high lipids diet(especially in the group M) were higher than ever(P<0.01),and that in the group P+A was obviously lower than that in the group A(P<0.01).Conclusion High lipids diet may due to the disorder of serum lipids and liver dysfunction.Combination therapy of probucol and atorvastatin can strengthen the effects on adjusting lipids,furthermore the effects are stronger than that of monotherapy.Combination therapy may reduce the hepatic damage that high lipids diet duo to,and decrease the hepatic negative effects of atorvastatin.